Table 1. HIV-1 Env V3 amino acid sequences with computational and biological prediction of co-receptor usage.
| Viruses | Env V3 region amino acid sequences a | 11/25ruleb | Geno2pheno(Subtype)c | PSSM(Scores) | U373 celllines | U87 celllines |
| HXB2 | CTRPNNNTRKRIRIQRGPGRAFVTIGK-IGNMRQAHC | |||||
| 1690 T | CTRPNNNTRKSINI–GPGRAFYATGEIIGDIRQAHC | NSI | R5 (B) | R5 (−8.88) | R5 | R5 |
| 1690 M | CTRPNNNTRKSINI–GPGRAFYATGEIIGDIRQAHC | NSI | R5 (B) | R5 (−8.88) | R5 | R5 |
| 1175 T | CTRPNNNTRKGIHI–GPGRAFYAR–IVGDIRQAHC | SI | X4 (B) | R5 (−8.88) | R5 | R5 |
| 1175 M | CTRPNNNTRKGIHI–GPGRAFYAR–IVGDIRQAHC | SI | X4 (B) | R5 (−8.88) | R5 | R5 |
a
10 to15 end-point diluted PCR products were directly sequenced from each virus, and the Env V3 regions of 1690 M, 1690 T, 1175 M and 1175 T, aligned to the HXB2 strain, were shown. The positions 11 and 25 of the Env V3 region are indicated in bold. “−” Represents a sequence gap.
b
Non-syncytia formation and syncytia formation HIV-1 are abbreviated as NSI and SI, respectively.
c
CCR5- and CXCR4- tropic viruses were indicated by R5 and X4, respectively.