Figure 3. Effect of various agonists on Trica5-HT1 in CHO-WTA11 cells.
(A) Receptor activation after stimulation with 100 µM of agonist, shown as the percentage of activation achieved with 100 µM of 5-HT (set at 100%). (B) Dose-dependent activation of Trica5-HT1 with synthetic 5-HT receptor agonists, shown as the percentage of activation achieved with 1 mM 5-HT (maximum response = 100%). Cells treated with BSA-medium only were used to define the basal level of 0%. Data represent the mean ± SEM of (A) three independent measurements (each performed in duplicate) and (B) seven independent measurements (each performed in duplicate) for 5-HT, or four independent measurements (three performed in triplicate, one performed in duplicate) for the synthetic agonists αm-5-HT, 5-MT, 5-CT and 8-OH-DPAT.