Figure 5. Effect of 5-HT on Trica5-HT1 in presence of different concentrations of antagonist.
Dose-dependent activation of Trica5-HT1 was measured in CHO-WTA11 cells with 1 nM –1 mM 5-HT in presence of 10 nM –1 mM antagonist: (A) prazosin, (B) methysergide and (C) methiothepin. Receptor activity is shown as the percentage of activation achieved with 1 mM of 5-HT (set at 100%). Cells treated with BSA-medium only were used to define the basal level of 0%. Data represent the mean ± SEM of three independent measurements (each performed in duplicate) for prazosin and methiothepin and two independent measurements (each performed in duplicate) for methysergide. NA, no antagonist.