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. 2013 May 10;10:140. doi: 10.1186/1743-422X-10-140

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Copyright © 2013 Ochieng et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Neighbour joining phylogenetic tree of nucleotide sequences of selected Orthobunyavirus isolates and reference sequences. The sequences of the Orthobunyavirus isolates under study (highlighted in yellow) were compared to other Orthobunyaviruses from the database [GenBank: EU564828.1, GenBank: EU564827.1, GenBank: JX857325.1, EMBL: AM709778.1, GenBank: AY729651.1, GenBank: JX846604.1, GenBank: GU206123.1, GenBank: AF362397.1]. The nucleocapsid region of these sequences and those of the selected Orthobunyaviruses under study were aligned using clustalW and the phylogenetic tree constructed using MEGA v5.05. Numbers on internal branches indicate bootstrap values for 1000 replicates. The Orthobunyaviruses from Kenya have clustered together while the reference sequences have also formed a separate clade. Bunyamwera and Ngari viruses have formed a cluster meaning they are closely related while Pongola virus seems to be different from the two viruses. GSA: Garissa; MGD: Magadi; TD: Tana Delta; S: site; PGAV: Pongola virus; BUNV: Bunyamwera virus; NRI: Ngari.