Table 1.

DNA sequences of the primers used in this study, their target genes/proteins and positions

	Virus	Gene/protein target	Primer sequence	Position	Reference
1.	Dengue	Structural polyprotein	D1; (5′-TCA ATA TGC TGA AAC GCG CGA GAA ACC G-3′)	38-65	[28]
	“	“	D2; (5′-TTG CAC CAA CAG TCA ATG TCT TCA GGT TC-3′)	455-483
2.	Alphavirus	NSP4	VIR 2052 F;(5′-TGG CGC TAT GAT GAA ATC TGG AAT GTT-3′)	6971–6997	[29]
	“	“	VIR 2052R; (5′-TAC GAT GTT GTC GTC GCC GAT GAA-3′)	7086–7109
3.	Flavivirus	NS5	FU 1; (5′- TAC AAC ATG ATG GGA AAG AGA GAG AA-3′)	9007-9032	[30]
	“	“	CFD2; (5′- GTG TCC CAG CCG GCG GTG TCA TCA GC-3′)	9308-9283
4.	bunyavirus	nucleocapsid protein	BCS82C; (5′-ATG ACT GAG TTG GAG TTT CAT GAT GTC GC-3′)	86-114	[31]
	“	“	BCS332V; (5′-TGT TCC TGT TGC CAG GAA AAT-3′)	309-329
5.	Ndumu	envelope (E1) gene	ND 124 F; (5′-CAC CCT AAA AGT GAC GTT-3′)	124-141	[30]
	“	“	ND 632R; (5′- ATT GCA GAT GGG ATA CCG-3′)	615-632
6.	West Nile	helicase	WN1315F; (5′-GCC AA TTT GCC TGC TCT AC-3′)	1315-1333	[32]
	“	“	WN1824R; (5′-CCA TCT TCA CTC TAC ACT TC-3′)	1824-1843
7.	Babanki	E1 envelope glycoprotein	Bab 3368 F; (5′- CAG CAG ATT GCG CGA CTG ACC-3′)	3368-3388	[30]
	“	“	Bab 4203R; (5′- GCT CAC GAT ATG GTC AGC AGG-3′)	4184-4203
8.	Batai	Polyprotein M segment	BATAIM3F; (5′-CCTGGGGAAGCATTGTGATTACT-3′)	852-874	[33]
	“	“	BATAIM3R; (5′-CTAGCCAGCGACTCTTGCCTTCC-3′)	2084-2103
9.	Sindbis	Non Structural Protein	SINV1; (5′-TTTAGCGGATCGGACAATTC-3′)	5194-5213	[34]
	“	“	SINV2; (5′-GCGGTGACGAACTCAGTAG-3′)	6482-6500
10.	RVF	Glycoprotein M gene	RVF1;(5′-GAC TAC CAG TCA GCT CAT TAC C-3′)	777-798	[34]
	“	“	RVF2;(5′-TGT GAA CAA TAG GCA TTG G-3′)	1309-1327
11.	Chikungunya/Onyon’gnyon’g	5′NTR	CHIK3F; (5′-CACACGTAGCCTACCAGTTTC-3′)	14-112	[35]
	“	“	CHIK3R; (5′-GCTGTCAGCGTCTATGTCCAC-3′)	“
	“	“	ONN3F; (5′-GATACACACACGCAGCTTACG-3′)	11-97
	“	“	ONN3R; (5′-TACATACACTGAATCCATGATGGG-3′)	“
12.	Yellow Fever	polyprotein	CAG; (5′- CGA GTT GCT AGG CAA TAA ACA CAT TTG GA-3)	43-71	[36]
	“	“	YF7; (5′- AAT GCT CCC TTT CCC AAA TA- 3′)	1293-1312