Table 1.
Overlapping sequences for homologous recombination
| |
Comparison to S. cerevisiae CEN.PK113-7D genome |
|||
|---|---|---|---|---|
| SHR-sequence | Sequence 5’ ➔ 3’ | Blast score | E-value | % GC content |
|
Optimized SHR-sequences |
|
|
|
|
| A |
ACTATATGTGAAGGCATGGCTATGGCACGGCAGACATTCCGCCAGATCATCAATAGGCAC |
28.3 |
1.3 |
50.0 |
| B |
CACCTTTCGAGAGGACGATGCCCGTGTCTAAATGATTCGACCAGCCTAAGAATGTTCAAC |
30.1 |
0.37 |
48.3 |
| C |
ACGTCTCACGGATCGTATATGCCGTAGCGACAATCTAAGAACTATGCGAGGACACGCTAG |
26.5 |
4.5 |
50.0 |
| D |
ACGCATCTACGACTGTGGGTCCCGTGGAGAAATGTATGAAACCCTGTATGGAGAGTGATT |
28.3 |
1.3 |
48.3 |
| F |
CATACGTTGAAACTACGGCAAAGGATTGGTCAGATCGCTTCATACAGGGAAAGTTCGGCA |
28.3 |
1.3 |
46.7 |
| G |
GCCAGAGGTATAGACATAGCCAGACCTACCTAATTGGTGCATCAGGTGGTCATGGCCCTT |
28.3 |
1.3 |
51.7 |
| H |
AGATTACTCTAACGCCTCAGCCATCATCGGTAATAGCTCGAATTGCTGAGAACCCGTGAC |
30.1 |
0.37 |
48.3 |
| I |
TATTCACGTAGACGGATAGGTATAGCCAGACATCAGCAGCATACTTCGGGAACCGTAGGC |
28.3 |
1.3 |
50.0 |
| J |
GGCCGTCATATACGCGAAGATGTCCAAGCAGGTAGAACACATAGTCTGAGCATCTCGTCG |
26.5 |
4.5 |
51.7 |
|
Endogenous sequences |
|
|
|
|
| A# |
GTCGACAACCCTTAATATAACTTCGTATAATGTATGCTATACGAAGTTATTAGGTCTAGA |
28.3 |
1.3 |
33.3 |
| B# |
GAGTGTTTAGAACATAATCAGTTTATCCATGGTCTATCTCTTCTTGTCGCTTTTTCTCCT |
28.3 |
1.3 |
36.7 |
| C# |
TTAATTTTAAATTTTTTTGGTAGTAAAAGATGCTTATATAAGGATTTCGTATTTATTGTT |
109 |
5e-25 |
18.3 |
| D# |
TAATATTTTTTCTTTTGAAAGTACTACCCACATCCGAACATTGCCACTTACATAGCGATG |
109 |
5e-25 |
35.0 |
| J# |
GAACAAAGTATTTAACGCACATGTATAAATATTGTATTAAAAGGGTACCTTTATAAATAT |
109 |
5e-25 |
23.3 |
| F# |
GAATAGTCTTTACACCCACAGTTTTTCGTGTGGCAGTTACTATATATTAGTAGGATATTC |
109 |
5e-25 |
35.0 |
| G# |
CTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTT |
30.1 |
0.37 |
35.0 |
| H# |
ACGTAGGATTATTATAACTCAAAAAAATGGCATTATTCTAAGTAAGTTAAATATCCGTAA |
109 |
5e-25 |
25.0 |
| I# | TTGGCAATTTTTTGCTCTTCTATATAACAGTTGAAATTTGAATAAGAACATCTTCTCAAA | 109 | 5e-25 | 26.7 |