Table 2.
Strains used in this study
| Strain | Relevant genotype | Source |
|---|---|---|
| CEN.PK113-7D |
MATα MAL2-8c SUC2 |
[26,33] |
| CEN.PK113-5D |
MATα ura3-52 MAL2-8c SUC2 |
[33] |
| IMX188 |
MATa ura3-52 his3-Δ1 leu2-3,112 MAL2-8c SUC2 glk1Δ::LoxP hxk1Δ::LoxP hxk2Δ::LoxP-KanMX-LoxP |
This study |
| IMX214 |
MATa ura3-52 his3-Δ1 leu2-3,112 MAL2-8c SUC2 glk1Δ::LoxP hxk1Δ::LoxP hxk2Δ::LoxP-KanMX-LoxP + PUDC074 |
This study |
| BY4743-Y23336 |
MATa/α; ura3Δ0/ura3Δ0; pgi1Δ::KanMX/PGI1 |
EUROSCARFa |
| BY4743-Y25893 |
MATa/α; ura3Δ0/ura3Δ0;pfk1Δ::KanMX/PFK1 |
EUROSCARFa |
| BY4743-Y20791 |
MATa/α; ura3Δ0/ura3Δ0; pfk2Δ::KanMX/PFK2 |
EUROSCARFa |
| BY4743-Y23986 |
MATa/α; ura3Δ0/ura3Δ0; tpi1Δ::KanMX/TPI1 |
EUROSCARFa |
| BY4743-Y24909 |
MATa/α; ura3Δ0/ura3Δ0; fba1Δ::KanMX/FBA1 |
EUROSCARFa |
| Spore from Y23336 + pUDC074 |
ura3Δ0; pgi1::kanMX pUDC074 (K.I.URA3(pUG72)TPI HXK2 FBA1 PGI PFK1 PFK2) |
This study |
| Spore from Y25893 + pUDC074 |
ura3Δ0; pfk1::kanMX pUDC074 (K.I.URA3(pUG72)TPI HXK2 FBA1 PGI PFK1 PFK2) |
This study |
| Spore from Y20791 + pUDC074 |
ura3Δ0; pfk2::kanMX pUDC074 (K.I.URA3(pUG72)TPI HXK2 FBA1 PGI PFK1 PFK2) |
This study |
| Spore from Y24909 + pUDC074 |
ura3Δ0; tpi1::kanMX pUDC074 (K.I.URA3(pUG72)TPI HXK2 FBA1 PGI PFK1 PFK2) |
This study |
| Spore from Y23986 + pUDC074 | ura3Δ0; fba1::kanMX pUDC074 (K.I.URA3(pUG72)TPI HXK2 FBA1 PGI PFK1 PFK2) | This study |