Table 3.
Primers used in this study
| Primers | Purification | Sequence 5’ ➔ 3’ |
|---|---|---|
| |
|
To add SHR-sequences |
| E.coli Rv +A |
HPLC |
GTGCCTATTGATGATCTGGCGGAATGTCTGCCGTGCCATAGCCATGCCTTCACATATAGTTGCGCGGAACCCCTATTTG |
| E.coli Fw +I |
HPLC |
TATTCACGTAGACGGATAGGTATAGCCAGACATCAGCAGCATACTTCGGGAACCGTAGGCGAGAGGCGGTTTGCGTATTGG |
| TPI1 Rv +H |
HPLC |
AGATTACTCTAACGCCTCAGCCATCATCGGTAATAGCTCGAATTGCTGAGAACCCGTGACTAGTGTGAGCGGGATTTAAACTGTG |
| TPI1 Fw +I |
HPLC |
GCCTACGGTTCCCGAAGTATGCTGCTGATGTCTGGCTATACCTATCCGTCTACGTGAATAGCGAAAATGACGCTTGCAGTG |
| FBA1 Rv +H |
HPLC |
GTCACGGGTTCTCAGCAATTCGAGCTATTACCGATGATGGCTGAGGCGTTAGAGTAATCTAAAATCTCAAAAATGTGTGGGTCATTACG |
| FBA1 Fw +G |
HPLC |
GCCAGAGGTATAGACATAGCCAGACCTACCTAATTGGTGCATCAGGTGGTCATGGCCCTTAGTGCATGACAAAAGATGAGCTAGG |
| Cen6 Ars4 Rv +G |
HPLC |
AAGGGCCATGACCACCTGATGCACCAATTAGGTAGGTCTGGCTATGTCTATACCTCTGGCGACGGATCGCTTGCCTGTAAC |
| Cen6 Ars4 Fw +F |
HPLC |
CATACGTTGAAACTACGGCAAAGGATTGGTCAGATCGCTTCATACAGGGAAAGTTCGGCAGTGCCACCTGGGTCCTTTTC |
| Cen6 Ars4 Rv +B |
HPLC |
CACCTTTCGAGAGGACGATGCCCGTGTCTAAATGATTCGACCAGCCTAAGAATGTTCAACGTGCCACCTGGGTCCTTTTC |
| Cen6 Ars4 Fw +C |
HPLC |
CTAGCGTGTCCTCGCATAGTTCTTAGATTGTCGCTACGGCATATACGATCCGTGAGACGTGACGGATCGCTTGCCTGTAAC |
| PFK2 Rv +F |
HPLC |
TGCCGAACTTTCCCTGTATGAAGCGATCTGACCAATCCTTTGCCGTAGTTTCAACGTATGATAGCCATTCTCTGCTGCTTTGTTG |
| PFK2 Fw +J |
HPLC |
GGCCGTCATATACGCGAAGATGTCCAAGCAGGTAGAACACATAGTCTGAGCATCTCGTCGGAGATCCGAGGGACGTTTATTGG |
| PFK1 Rv +D |
HPLC |
ACGCATCTACGACTGTGGGTCCCGTGGAGAAATGTATGAAACCCTGTATGGAGAGTGATTTCGAGATTCCTCAATCCATACACCATTATAG |
| PFK1 Fw +J |
HPLC |
CGACGAGATGCTCAGACTATGTGTTCTACCTGCTTGGACATCTTCGCGTATATGACGGCCTGTCGTCTTCGTGAACCATTGTC |
| PGI1 Rv +D |
HPLC |
AATCACTCTCCATACAGGGTTTCATACATTTCTCCACGGGACCCACAGTCGTAGATGCGTCTGAAGAAGGCATACTACGCCAAG |
| PGI1 Fw +C |
HPLC |
ACGTCTCACGGATCGTATATGCCGTAGCGACAATCTAAGAACTATGCGAGGACACGCTAGTTCGCGACACAATAAAGTCTTCACG |
| HXK2 Rv +C |
HPLC |
CTAGCGTGTCCTCGCATAGTTCTTAGATTGTCGCTACGGCATATACGATCCGTGAGACGTGCAAGAGAAAAAAACGAGCAATTGTTAAAAG |
| HXK2 Fw +B |
HPLC |
CACCTTTCGAGAGGACGATGCCCGTGTCTAAATGATTCGACCAGCCTAAGAATGTTCAACGACGGCACCGGGAAATAAACC |
| URA3K.l. Rv +B |
HPLC |
GTTGAACATTCTTAGGCTGGTCGAATCATTTAGACACGGGCATCGTCCTCTCGAAAGGTGCTCAGAAGCTCATCGAACTGTCATC |
| URA3K.l. Fw + A |
HPLC |
ACTATATGTGAAGGCATGGCTATGGCACGGCAGACATTCCGCCAGATCATCAATAGGCACGATCCCAATACAACAGATCACGTGATC |
| FUS1 |
HPLC |
ACTATATGTGAAGGCATGGCTATGG |
| FUS2 |
HPLC |
CTAGCGTGTCCTCGCATAGTTC |
| Amp-rv + A-ctrl |
HPLC |
TCTAGACCTAATAACTTCGTATAGCATACATTATACGAAGTTATATTAAGGGTTGTCGACTGCGCGGAACCCCTATTTG |
| Amp-fw + I-ctrl |
HPLC |
TTGGCAATTTTTTGCTCTTCTATATAACAGTTGAAATTTGAATAAGAACATCTTCTCAAAGAGAGGCGGTTTGCGTATTGG |
| CEN/6ARS4-fw + F-ctrl |
HPLC |
GAATAGTCTTTACACCCACAGTTTTTCGTGTGGCAGTTACTATATATTAGTAGGATATTCGTGCCACCTGGGTCCTTTTC |
| CEN6ARS4-rv + G-ctrl |
HPLC |
AAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGGACGGATCGCTTGCCTGTAAC |
| FBA1-fw + G-ctrl |
HPLC |
CTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTAGTGCATGACAAAAGATGAGCTAGG |
| FBA1-rv + H-ctrl |
HPLC |
TTACGGATATTTAACTTACTTAGAATAATGCCATTTTTTTGAGTTATAATAATCCTACGTAAAATCTCAAAAATGTGTGGGTCATTACG |
| HXK2-fw + B-ctrl |
HPLC |
GAGTGTTTAGAACATAATCAGTTTATCCATGGTCTATCTCTTCTTGTCGCTTTTTCTCCTGACGGCACCGGGAAATAAACC |
| HXK2-rv + C-ctrl |
HPLC |
AACAATAAATACGAAATCCTTATATAAGCATCTTTTACTACCAAAAAAATTTAAAATTAAGCAAGAGAAAAAAACGAGCAATTGTTAAAAG |
| K.l.URA3 -fw + A-ctrl |
HPLC |
GTCGACAACCCTTAATATAACTTCGTATAATGTATGCTATACGAAGTTATTAGGTCTAGAGATCCCAATACAACAGATCACGTGATC |
| K.l.URA3-rv + B-ctrl |
HPLC |
AGGAGAAAAAGCGACAAGAAGAGATAGACCATGGATAAACTGATTATGTTCTAAACACTCCTCAGAAGCTCATCGAACTGTCATC |
| PFK1-fw + J-ctrl |
HPLC |
ATATTTATAAAGGTACCCTTTTAATACAATATTTATACATGTGCGTTAAATACTTTGTTCTGTCGTCTTCGTGAACCATTGTC |
| PFK1-rv + D-ctrl |
HPLC |
TAATATTTTTTCTTTTGAAAGTACTACCCACATCCGAACATTGCCACTTACATAGCGATGTCGAGATTCCTCAATCCATACACCATTATAG |
| PFK2-fw + J-ctrl |
HPLC |
GAACAAAGTATTTAACGCACATGTATAAATATTGTATTAAAAGGGTACCTTTATAAATATGAGATCCGAGGGACGTTTATTGG |
| PFK2-rv + F-ctrl |
HPLC |
GAATATCCTACTAATATATAGTAACTGCCACACGAAAAACTGTGGGTGTAAAGACTATTCATAGCCATTCTCTGCTGCTTTGTTG |
| PGI-fw + C-ctrl |
HPLC |
TTAATTTTAAATTTTTTTGGTAGTAAAAGATGCTTATATAAGGATTTCGTATTTATTGTTTTCGCGACACAATAAAGTCTTCACG |
| PGI-rv + D-ctrl |
HPLC |
CATCGCTATGTAAGTGGCAATGTTCGGATGTGGGTAGTACTTTCAAAAGAAAAAATATTACTGAAGAAGGCATACTACGCCAAG |
| TPI-fw + I-ctrl |
HPLC |
TTTGAGAAGATGTTCTTATTCAAATTTCAACTGTTATATAGAAGAGCAAAAAATTGCCAAGCGAAAATGACGCTTGCAGTG |
| TPI-rv + H-ctrl |
HPLC |
ACGTAGGATTATTATAACTCAAAAAAATGGCATTATTCTAAGTAAGTTAAATATCCGTAATAGTGTGAGCGGGATTTAAACTGTG |
| |
|
For multipex PCR |
| A Ctrl Fw |
Desalted |
AAATAAACAAATAGGGGTTCCGC |
| A Ctrl Rv |
Desalted |
GCAACACTCACTTCAACTTCATC |
| B Ctrl Fw |
Desalted |
TTACCACCATCCAATGCAGAC |
| B Ctrl Rv |
Desalted |
ACGGAATAGAACACGATATTTGC |
| C Ctrl Fw |
Desalted |
TCACGGGATTTATTCGTGACG |
| C Ctrl Rv |
Desalted |
GCGTCCAAGTAACTACATTATGTG |
| D Ctrl Fw |
Desalted |
ACTCGCCTCTAACCCCACG |
| D Ctrl Rv |
Desalted |
ACGGACTATAATGGTGTATGGATTG |
| J Ctrl Fw |
Desalted |
GCTTAATCTGCGTTGACAATGG |
| J Ctrl Rv |
Desalted |
CAATAAACGTCCCTCGGATCTC |
| F Ctrl Fw |
Desalted |
GACGCCATTTGGAACGAAAAAAAG |
| F Ctrl Rv |
Desalted |
ATAGCACGTGATGAAAAGGAC |
| G Ctrl Fw |
Desalted |
GCGTGTAAGTTACAGGCAAGC |
| G Ctrl Rv |
Desalted |
GCTCTTTTCTTCTGAAGGTCAATG |
| H Ctrl Fw |
Desalted |
GTTACGTGCTCAGTTGTTAGATATG |
| H Ctrl Rv |
Desalted |
GCAGAAGTGTCTGAATGTATTAAGG |
| I Ctrl Fw |
Desalted |
TGAGCCACTTAAATTTCGTGAATG |
| I Ctrl Rv | Desalted | GCCTTTGAGTGAGCTGATACC |