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. 2013 May 11;2:14. doi: 10.1186/2162-3619-2-14

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Copyright © 2013 Prinz et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expanded γδ T cells in the patient’s blood are Vδ1⁺γδ T cells. Further flow cytometric characterization of donor γδ T cells recovered from the patient 9 months after transplantation. Ficoll-purified blood samples were stained with PE-Cy7-conjugated anti CD3 (BD bioscience, clone SK7), PC5-conjugated Vδ9 (Beckman Coulter cat. # PNA63663), and FITC-conjugated Vδ1 (Thermo Scientific, clone TS8.2, cat. # EN-TCR2730). Numbers next to quadrants indicate percentage of gated Vδ9⁺ (left panel) or Vδ1⁺ (right panel) γδ T cells among all CD3⁺ lymphocytes.