Figure 6. ChIP analyses revealing reduced binding of Sp1 and the status of histone acetylation associated with LEDGF promoter. Left panel shows Sp1 binding to its responsive cis-elements (B), association and recruitment of site-specifically modified H3 (C), H2B (D), and HDAC1(E) to the Sp1 binding sites within CpG island of LEDGF gene promoter in LECs exposed or unexposed to UVB radiation. Right panel represents control; DNA selected beyond Sp1 binding sites is shown. ChIP assay was performed. Also, amplification of chromatin extract before precipitation is shown as control (input, A)