Table 5. Mean methylated fraction of CpG sites in the RFC1 gene in subjects with low or high plasma tHcy concentration.
| |
|
Low tHcy |
High tHcy |
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CpG site* | Mean | SD | N | Mean | SD | N | p | ||||
| 5′shore |
8 |
60.5 |
6.7 |
23 |
53.0 |
12.5 |
24 |
0.037 |
|||
| |
9 |
72.5 |
6.3 |
23 |
64.2 |
13.2 |
24 |
0.022 |
|||
| |
10 |
75.0 |
5.4 |
23 |
64.4 |
15.7 |
24 |
0.009 |
|||
| |
13 |
73.0 |
5.5 |
23 |
64.5 |
13.6 |
24 |
0.017 |
|||
| |
15 |
31.5 |
5.6 |
23 |
26.1 |
7.8 |
24 |
0.030 |
|||
| |
16 |
56.0 |
7.0 |
23 |
47.8 |
8.9 |
24 |
0.002 |
|||
| |
17 |
21.7 |
4.2 |
23 |
16.6 |
3.9 |
24 |
0.000 |
|||
| |
|
|
|
|
|
|
|
|
|||
| CGI 1 |
18 |
20.9 |
6.0 |
24 |
15.5 |
4.4 |
24 |
0.005 |
|||
| |
21 |
10.5 |
3.5 |
24 |
7.5 |
1.5 |
24 |
0.024 |
|||
| |
25 |
23.3 |
3.2 |
24 |
20.1 |
4.6 |
24 |
0.038 |
|||
| |
|
|
|
|
|
|
|
|
|||
| 3′ shore |
38 |
98.3 |
1.8 |
24 |
97.1 |
1.7 |
24 |
0.029 |
|||
| |
43 |
74.4 |
5.9 |
23 |
78.5 |
2.6 |
22 |
0.010 |
|||
| |
46 |
80.0 |
1.4 |
23 |
81.5 |
1.8 |
22 |
0.032 |
|||
| |
|
|
|
|
|
|
|
|
|||
| CGI 2 |
50 |
99.3 |
1.2 |
22 |
98.5 |
1.4 |
24 |
0.045 |
|||
| |
52 |
98.5 |
1.4 |
22 |
97.2 |
1.7 |
24 |
0.007 |
|||
| |
53 |
77.1 |
2.2 |
22 |
78.5 |
1.7 |
24 |
0.020 |
|||
| 55 | 97.3 | 2.2 | 22 | 95.9 | 1.9 | 24 | 0.025 | ||||
*
CpG sites included in the methylation assays, see Table 3. Only sites showing statistically significant differences are show in this table. P, post-hoc significance of difference between mean values in leukocytes from subjects with low or high tHcy, respectively.