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. 2013 Feb 15;8(3):303–316. doi: 10.4161/epi.23988

Table 7. Methylated fraction of CpG sites in the RFC1 gene in blood leukocytes form subjects with low and high tHcy according to genotype.

 
  
 Low tHcy
  
 High tHcy
  
  
  
CpG site Genotype Mean SD N p1 Mean SD N p1 p2  
 
  
  
  
  
  
  
  
  
  
  
  
CpG 3
 GG
 89.3
 0.5
 6
  
 89.9
 0.6
 8
  
  
  
 
 GA
 89.3
 0.7
 12
  
 89.8
 0.8
 12
  
  
  
 
 AA
 89.0
 0.8
 4
 0.656
 90.0
 0.8
 4
 0.823
 0.006
 
CpG 6
 GG
 85.3
 0.8
 6
  
 83.7
 1.0
 7
  
  
  
 
 GA
 84.7
 1.3
 12
  
 85.0
 1.1
 12
  
  
  
 
 AA
 85.3
 0.5
 4
 0.415
 84.3
 1.5
 4
 0.079
 0.046
 
CpG 8
 GG
 56.8
 8.6
 6
  
 50.3
 11.4
 8
  
  
  
 
 GA
 61.0
 6.2
 12
  
 56.3
 8.5
 12
  
  
  
 
 AA
 61.0
 1.0
 3
 0.448
 48.5
 23.2
 4
 0.434
 0.029
 
CpG 9
 GG
 68.0
 7.6
 6
  
 60.4
 11.2
 8
  
  
  
 
 GA
 73.2
 5.2
 12
  
 68.1
 8.8
 12
  
  
  
 
 AA
 73.3
 0.6
 3
 0.195
 60.3
 25.3
 4
 0.372
 0.020
 
CpG 10
 GG
 72.2
 7.6
 6
  
 58.8
 16.1
 8
  
  
  
 
 GA
 75.6
 4.5
 12
  
 69.5
 9.4
 12
  
  
  
 
 AA
 76.0
 3.6
 3
 0.432
 60.5
 27.4
 4
 0.291
 0.007
 
CpG 11
 GG
 83.5
 4.9
 6
  
 80.3
 10.5
 8
  
  
  
 
 GA
 85.5
 4.5
 12
  
 82.6
 6.8
 12
  
  
  
 
 AA
 86.3
 1.5
 3
 0.578
 74.3
 21.5
 4
 0.454
 0.049
 
CpG 13
 GG
 69.5
 6.4
 6
  
 59.5
 14.5
 8
  
  
  
 
 GA
 73.5
 5.3
 12
  
 68.8
 7.9
 12
  
  
  
 
 AA
 74.0
 0.0
 3
 0.307
 61.3
 23.1
 4
 0.293
 0.014
 
CpG 15
 GG
 26.6
 2.1
 5
  
 26.1
 7.1
 8
  
  
  
 
 GA
 33.2
 6.3
 12
  
 27.8
 7.5
 12
  
  
  
 
 AA
 32.5
 5.4
 4
 0.099
 21.3
 10.2
 4
 0.370
 0.016
 
CpG 16
 GG
 52.2
 4.4
 5
  
 46.9
 7.8
 8
  
  
  
 
 GA
 56.3
 7.6
 12
  
 50.4
 7.0
 12
  
  
  
 
 AA
 59.5
 8.7
 4
 0.332
 41.8
 14.4
 4
 0.233
 0.001
 
CpG 17
 GG
 19.2
 1.6
 5
  
 16.1
 2.9
 8
  
  
  
 
 GA
 22.3
 4.9
 12
  
 18.1
 3.7
 12
  
  
  
 
 AA
 22.3
 4.4
 4
 0.407
 13.3
 4.3
 4
 0.080
 0.000
 
CpG 18
 GG
 16.2
 2.6
 6
  
 15.1
 4.1
 8
  
  
  
 
 GA
 22.3
 6.4
 12
  
 17.4
 2.8
 12
  
  
  
 
 AA
 23.5
 7.2
 4
 0.089
 10.8
 5.7
 4
 0.021
0.000
 
CpG 19
 GG
 8.8
 1.6
 6
  
 8.5
 2.2
 8
  
  
  
 
 GA
 11.3
 5.9
 12
  
 9.2
 1.7
 12
  
  
  
 
 AA
 13.5
 4.8
 4
 0.350
 6.3
 2.8
 4
 0.070
 0.011
 
CpG 20
 GG
 8.0
 1.4
 6
  
 7.6
 2.0
 8
  
  
  
 
 GA
 10.1
 5.5
 12
  
 8.5
 1.9
 12
  
  
  
 
 AA
 12.3
 4.4
 4
 0.370
 5.3
 2.8
 4
 0.042
0.012
 
CpG 21
 GG
 8.2
 1.2
 6
  
 7.4
 1.2
 8
  
  
  
 
 GA
 11.2
 4.1
 12
  
 8.3
 1.1
 12
  
  
  
 
 AA
 11.8
 3.8
 4
 0.190
 5.8
 1.9
 4
 0.008
0.000
 
CpG 22
 GG
 3.3
 0.5
 6
  
 3.3
 1.6
 8
  
  
  
 
 GA
 5.6
 3.1
 12
  
 4.3
 0.9
 12
  
  
  
 
 AA
 6.5
 2.4
 4
 0.132
 2.8
 1.3
 4
 0.052
 0.012
 
CpG 24
 GG
 17.2
 3.0
 6
  
 14.8
 3.5
 8
  
  
  
 
 GA
 21.2
 2.8
 12
  
 18.8
 4.8
 12
  
  
  
 
 AA
 19.3
 2.1
 4
 0.028
14.5
 3.3
 4
 0.081
 0.009
 
CpG 25
 GG
 21.3
 2.9
 6
  
 18.1
 4.8
 8
  
  
  
 
 GA
 24.3
 3.4
 12
  
 21.6
 4.1
 12
  
  
  
 
 AA
 22.8
 1.7
 4
 0.186
 19.8
 5.1
 4
 0.265
 0.026
 
CpG 31
 GG
 80.5
 3.3
 6
  
 78.9
 4.6
 8
  
  
  
 
 GA
 83.0
 1.1
 12
  
 81.8
 3.7
 12
  
  
  
 
 AA
 84.3
 2.1
 4
 0.025
80.5
 4.5
 4
 0.313
 0.051
  
CpG 34
 GG
 91.0
 1.2
 4
  
 90.0
 1.3
 6
  
  
  
 
 GA
 92.8
 0.9
 10
  
 92.2
 1.4
 11
  
  
  
 
 AA
 93.3
 1.0
 4
 0.010
93.3
 1.9
 4
 0.006
0.232
  
CpG 38
 GG
 98.7
 1.2
 6
  
 96.6
 2.0
 8
  
  
  
 
 GA
 98.2
 1.7
 12
  
 97.4
 1.7
 12
  
  
  
 
 AA
 98.5
 2.4
 4
 0.833
 97.0
 1.6
 4
 0.627
 0.017
 
CpG 43
 GG
 73.4
 8.3
 5
  
 77.8
 2.9
 6
  
  
  
 
 GA
 76.7
 4.0
 12
  
 78.6
 2.7
 12
  
  
  
 
 AA
 71.3
 6.4
 4
 0.228
 79.3
 1.7
 4
 0.703
 0.003
 
CpG 46
 GG
 80.4
 1.8
 5
  
 81.0
 0.6
 6
  
  
  
 
 GA
 79.8
 1.4
 12
  
 81.1
 1.4
 12
  
  
  
 
 AA
 80.5
 1.3
 4
 0.583
 83.3
 3.3
 4
 0.093
 0.007
 
CpG 50
 GG
 99.7
 0.8
 6
  
 98.4
 1.4
 8
  
  
  
 
 GA
 99.2
 1.3
 12
  
 98.7
 1.6
 12
  
  
  
 
 AA
 99.0
 1.4
 4
 0.646
 98.0
 1.4
 4
 0.730
 0.044
 
CpG 51
 GG
 99.5
 0.5
 6
  
 98.0
 1.3
 8
  
  
  
 
 GA
 98.6
 1.4
 12
  
 98.2
 1.6
 12
  
  
  
 
 AA
 99.5
 1.0
 4
 0.230
 98.8
 1.5
 4
 0.710
 0.052
  
CpG 52
 GG
 98.0
 1.7
 6
  
 97.0
 1.5
 8
  
  
  
 
 GA
 98.4
 1.4
 12
  
 97.4
 1.8
 12
  
  
  
 
 AA
 99.3
 1.0
 4
 0.402
 96.8
 1.7
 4
 0.756
 0.006
 
CpG 53
 GG
 76.8
 2.9
 6
  
 78.1
 1.5
 8
  
  
  
 
 GA
 77.3
 2.1
 12
  
 78.6
 1.8
 12
  
  
  
 
 AA
 77.0
 1.6
 4
 0.902
 79.0
 1.4
 4
 0.675
 0.024
 
CpG 54
 GG
 76.2
 2.6
 6
  
 77.9
 1.1
 8
  
  
  
 
 GA
 38.8
 1.9
 12
  
 39.2
 1.6
 12
  
  
  
 
 AA
 3.3
 1.0
 4
 0.000
2.5
 0.6
 4
 0.000
0.417
  
CpG 55
 GG
 98.2
 1.9
 6
  
 96.3
 2.4
 8
  
  
  
 
 GA
 97.0
 2.5
 12
  
 96.0
 1.7
 12
  
  
  
  AA 97.0 1.6 4 0.568 95.0 1.4 4 0.565 0.021  

p1 difference between mean methylated fraction in subjects with different RFC1 80G > A genotypes. p2 difference between mean methylated fraction in subjects with high or low tHcy concentration, adjusted for RFC1 80G > A genotype. Please refer to the Statistics section for details of the multivariate model used.