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. 2013 May 31;8(5):e65701. doi: 10.1371/journal.pone.0065701

Figure 5. SPP1 expression in the developing macaque monkey.

Figure 5

A–D: Nissl-stained and adjacent sections showing the distribution of SPP1 mRNA-positive neurons in the primary motor cortex (M1) of macaque monkey at postnatal day 182 (P182; A, B) and P730 (C, D). E, F: High-magnification photomicrographs of SPP1 mRNA-positive neurons in layer V pyramidal neurons of the macaque monkey M1 at P182 (E) and P730 (F). Arrows and double arrowheads indicate neurons with the cell body size mainly showing intense signals at P182 and P730, respectively. G, H: Nissl-stained and adjacent sections hybridized with the SPP1 antisense probe in the macaque monkey M1 at P10. I: Scattergram showing the relationship between the average size of Nissl-stained neurons with the largest cell bodies and that of SPP1-positive neurons in the macaque monkey at each developmental stage. ***P<0.0001, according to linear regression analysis. J: Scattergram showing the logarithmic correlation between age and the density of SPP1 mRNA-positive neurons in the developing macaque monkey M1. ***P<0.0001, according to Spearman's coefficient of rank correlation. Diamonds represent data points from each monkey. II–VI, layers II–VI of M1. Scale bars  = 200 µm in A–D, G, H; 50 µm in E, F.