Figure 7. E2F4 acts as a potent regulator of LTBP4S expression in combination with other regulatory elements.

HEK293 cells were transiently transfected with pGL4.23-LTBP4L or pGL4.23-LTBP4S and a Renilla luciferase expression vector for normalization. Cells were also co-transfected with different concentrations of an E2F4 expression vector. After 24 h cells were lysed and luciferase activities were determined. Experiments were repeated at least 3 times and the mean value was calculated. Data are presented ± standard deviation.