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| Purification step | Total activity (U) |
Specific activity (U/mg) | Purification fold | Yield (%) |
|---|---|---|---|---|
| Crude extract |
34,410 |
14.9 |
1 |
100 |
| Affinity chromatography (IMAC) |
21,930 |
145.0 |
9.7 |
63.7 |
| Cold storage* | 21,150 | 281.2 | 18.9 | 61.5 |
The MLE was produced from 1.5 L fermentation broth. Values reported are the mean of two independent measurements. *After removing precipitated (inactive) protein by centrifugation.
