Table 2. Side activities of the recombinant malolactic enzyme.
| Substrate (10 mM) |
Cofactor | Mn2+ (mM) | Specific activity (U/mg protein) | Activity assay for |
|---|---|---|---|---|
| L-malic acid |
0.5 mM NAD+ |
- |
0.8 |
L-MDH |
| L-malic acid |
0.5 mM NAD+ |
0.1 |
1.2 |
ME |
| Oxaloacetic acid |
0.5 mM NADH |
- |
ND |
L-MDH |
| Oxaloacetic acid |
0.5 mM NADH |
0.1 |
ND |
(L-MDH) |
| L-Lactic acid |
0.5 mM NAD+ |
- |
ND |
L-LDH |
| L-Lactic acid |
0.5 mM NAD+ |
0.1 |
ND |
(L-LDH) |
| Pyruvic acid |
0.5 mM NADH |
- |
1.2 |
L-LDH |
| Pyruvic acid | 0.5 mM NADH | 0.1 | 4.5 | (L-LDH) |
The enzyme activities for malic enzyme (ME), L-malate dehydrogenase (L-MDH) and L-lactate dehydrogenase (L-LDH) were determined. Values reported are the mean of two independent experiments. ND, not detectable.