Table 3. Properties of the growth-coupled overproducer designs under mixotrophic conditions.
| |
Mixotrophic conditions |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Light limiting state (0.145) | Carbon limiting state (0.238) | |||||||||
| Metabolite |
Maximun production rate |
Number of knockouts |
Growth rate |
Production rate (mmol.gDW−1h−1) |
BPCY |
Maximun production rate |
Number Knockouts |
Growth rate |
Production rate |
BPCY |
| (mmol.gDW−1h−1) |
(h−1) |
(mmol.gDW−1h−1) |
(h−1) |
(mmol.gDW−1h−1) |
||||||
| Fumarate (4 C) |
2.351 |
6 |
0.061 |
0.88 |
0.0533 |
2.303 |
6 |
0.061 |
0.88 |
0.0533 |
| Ethanol (2 C) |
3.092 |
10 |
0.091 |
0.487 |
0.0441 |
4.607 |
7 |
0.037 |
0.682 |
0.0256 |
| 1-Butanol (4 C) |
1.546 |
5 |
0.038 |
0.591 |
0.0222 |
2.303 |
5 |
0.038 |
0.591 |
0.0222 |
| Sucrose (12 C) |
0.732 |
5 |
0.03 |
0.295 |
0.0088 |
0.767 |
5 |
0.03 |
0.295 |
0.0088 |
| Lactate (3 C) |
3.085 |
6 |
0.029 |
1.008 |
0.0291 |
3.071 |
6 |
0.029 |
1.008 |
0.0291 |
| H2 | 18.554 | 11 | 0.112 | 2.897 | 0.3248 | 35.452 | 8 | 0.156 | 16.12 | 2.507 |
LLS, Light limiting state; CLS, Carbon limiting state; BPCY, Biomass-product coupled yield. Numbers inside parenthesis represent the wild-type growth rate.