Table 2. Small bowel resection alters the composition of the colonic microbiota over time. The composition of the colonic microbiota is altered two and six weeks post-surgery in the small bowel resection (SBR) group. Values are relative proportions of operational taxonomic units (OTUs) ± SEM, n = 5−6/group/time-point. Statistical significance was determined using a general linear model ANOVA; *p < 0.05; **p < 0.01; ***p < 0.001 compared with non-operation control (NOC) group; †p < 0.05, ††p < 0.01, †††p < 0.001 compared with sham group.
| Weeks post-surgery | Week 2 | Week 6 | ||||
|---|---|---|---|---|---|---|
|
Group |
NOC |
Sham |
SBR |
NOC |
Sham |
SBR |
|
Phylum: |
|
|
|
|
|
|
| Firmicutes |
52.6 ± 5.0 |
52.1 ± 10.6 |
68.5 ± 6.1 |
61.7 ± 6.5 |
67.7 ± 3.6 |
68.2 ± 4.2 |
| Proteobacteria |
27.8 ± 5.1 |
23.1 ± 14.6 |
11.9 ± 4.6 |
10.6 ± 3.3 |
6.0 ± 0.6 |
6.2 ± 1.8 |
| Bacteroidetes |
17.9 ± 2.5 |
19.1 ± 5.8 |
10.1 ± 1.7 |
22.3 ± 2.6 |
23.2 ± 1.9 |
10.0 ± 1.6**†† |
| Fusobacteria |
0.9 ± 0.4 |
3.8 ± 3.1 |
8.5 ± 2.7 |
4.7 ± 2.3 |
2.8 ± 1.4 |
15.4 ± 2.4**†† |
| Actinobacteria |
0.7 ± 0.2 |
2.0 ± 1.5 |
1.0 ± 0.4 |
0.7 ± 0.2 |
0.3 ± 0.1 |
0.3 ± 0.1 |
|
Family: |
|
|
|
|
|
|
| Enterobacteriaceae |
31.8 ± 6.9 |
5.8 ± 2.5** |
1.0 ± 0.5*** |
6.7 ± 2.7 |
1.9 ± 0.7 |
4.0 ± 2.1 |
| Ruminococcaceae |
28.1 ± 3.1 |
27.0 ± 4.2 |
5.2 ± 2.5**† |
41.5 ± 5.2 |
36.1 ± 4.1 |
3.9 ± 1.6***††† |
| Veillonellaceae |
19.5 ± 4.9 |
36.7 ± 8.4 |
70.5 ± 7.1**† |
9.9 ± 2.9 |
28.0 ± 6.5 |
64.2 ± 6.6***††† |
| Peptostreptococcaceae |
4.6 ± 1.5 |
2.1 ± 1.5 |
0 |
2.8 ± 0.6 |
3.7 ± 1.4 |
0*† |
| Prevotellaceae |
3.9 ± 0.6 |
14.4 ± 2.8* |
7.1 ± 0.5 |
2.0 ± 0.9 |
9.7 ± 1.8** |
5.8 ± 0.7** |
| Bacteroidaceae |
3.5 ± 0.8 |
1.1 ± 0.6 |
2.4 ± 1.1 |
9.0 ± 2.1 |
6.0 ± 0.9 |
2.9 ± 1.2 |
| Erysipelotrichaceae |
2.5 ± 0.9 |
1.4 ± 0.7 |
0.4 ± 0.2 |
10.0 ± 6.0 |
3.8 ± 1.3 |
0 |
| Clostridiaceae |
2.1 ± 0.5 |
1.0 ± 0.5 |
0 |
0.7 ± 0.1 |
2.7 ± 1.3 |
0.1 ± 0.0 |
| Lactobacillaceae |
1.5 ± 0.6 |
2.1 ± 1.1 |
1.2 ± 0.3 |
2.9 ± 0.8 |
0.2 ± 0.1* |
0.8 ± 0.4 |
| Fusobacteriaceae |
1.4 ± 0.6 |
7.5 ± 3.9 |
11.9 ± 4.1 |
8.3 ± 4.3 |
4.8 ± 2.5 |
18.2 ± 3.0**†† |
| Peptococcaceae |
1.1 ± 0.5 |
0.9 ± 0.3 |
0.4 ± 0.4 |
6.3 ± 1.9 |
3.2 ± 0.7 |
0** |
|
Genus: |
|
|
|
|
|
|
| Enterobacteriaceae family members |
48.8 ± 8.7 |
9.4 ± 3.7*** |
1.4 ± 0.7*** |
11.6 ± 2.8 |
3.6 ± 1.1 |
5.7 ± 2.9 |
| Bacteroides |
5.8 ± 1.3 |
2.1 ± 1.1 |
3.5 ± 1.5 |
21.2 ± 3.2 |
11.5 ± 1.6 |
4.3 ± 1.7 |
| Parabacteroides |
5.1 ± 1.2 |
10.9 ± 3.3 |
1.6 ± 1.1 |
19.2 ± 4.5 |
10.9 ± 3.4 |
0.6 ± 0.2* |
| Prevotella |
6.7 ± 1.6 |
23.2 ± 4.5* |
10.4 ± 1.2 |
3.1 ± 1.5 |
18.9 ± 3.4** |
9.6 ± 0.9* |
| Clostridium |
3.4 ± 0.7 |
1.8 ± 0.9 |
0 |
1.8 ± 0.6 |
5.1 ± 2.6 |
0.1 ± 0.1† |
| Fusobacterium |
2.7 ± 1.4 |
12.4 ± 6.6 |
17.7 ± 6.2 |
11.9 ± 5.1 |
9.0 ± 4.8 |
29.9 ± 4.3**†† |
| Megasphaera |
17.1 ± 6.0 |
27.3 ± 4.4 |
31.4 ± 6.1 |
6.2 ± 2.5 |
23.5 ± 7.7 |
18.3 ± 3.0* |
| Acidaminococcus |
4.5 ± 1.2 |
8.0 ± 2.8 |
31.7 ± 6.7**†† |
3.6 ± 2.3 |
11.1 ± 6.5 |
30.2 ± 5.1***†† |
| Peptococcus |
1.9 ± 0.9 |
1.5 ± 0.6 |
0.7 ± 0.6 |
14.9 ± 5.7 |
6.1 ± 1.7 |
0.1 ± 0.1 |
| Lactobacillus | 4.0 ± 1.8 | 3.4 ± 1.8 | 1.7 ± 0.4 | 6.4 ± 1.9 | 0.4 ± 0.2* | 1.3 ± 0.6 |