Table 2. Quantitative real time PCR primers for bacterial group-specific16S rDNA.

Bacterial Target Group	Primer Sequence (5′-3′)	Annealing Temp (°C)	Reference
Eubacteria	ACT CCT ACG GGA GGC AGC AGT	62.6	46
	ATT ACC GCG GCT GCT GGC	61.7
Bacteroides	GAG AGG AAG GTC CCC CAC	59.2	16
	CGC TAC TTG GCT GGT TCA G	56.0
Mouse Intestinal Bacteroides (MIB)	CCA GCA GCC GCG GTA ATA	57.9	46
	CGC ATT CCG CAT ACT TCT C	53.7
Bacillus	GCG GCG TGC CTA ATA CAT GC	59.4	16
	CTT CAT CAC TCA CGC GGC GT	60.4
Lactobacillus	AGC AGT AGG GAA TCT TCC A	52.7	46
	CAC CGC TAC ACA TGG AG	52.8
Clostidium coccoides (cluster XIVa)	ACT CCT ACG GGA GGC AGC	59.4	46
	GCT TCT TAG TCA GGT ACC GTC AT	56.2
Clostridium leptum (cluster IV)	CCT TCC GTG CCG SAG TTA	57.6	47
	GAA TTA AAC CAC ATA CTC CAC TGC TT	55.5
SFB	GAC GCT GAG GCA TGA GAG CAT	59.7	46
	GAC GGC ACG GAT TGT TAT TCA	55.5
Bifidobacterium	CGG GTG AGT AAT GCG TGA CC	58.3	47
	TGA TAG GAC GCG ACC CCA	58.4
Enterobacteriaceae	GTG CCA GCM GCC GCG GTA A	65.2	46
	GCC TCA AGG GCA CAA CCT CCA AG	62.6