Table 1. Effects of IFNA2c on cell cycle, induction of autophagy and inhibition of proliferation.
| Cell line | Cell cycle analysis (% of total cells) | Autophagy activity factor (AAF) (mean ± SD) | Growth inhibition (%) (mean ± SD) |
||||
|---|---|---|---|---|---|---|---|
| |
|
Apoptotic |
G0/G1 |
S |
G2/M |
|
|
| Group 1 |
Daudi |
0.1 ± 0.1 |
42.6 ± 2.5 |
20.2 ± 1.0 |
24.8 ± 2.8 |
27.8 ± 8.8 |
66 ± 15** |
| Daudi + IFNA2c |
0.6 ± 1.0 |
53.9 ± 3.8 |
15.3 ± 3.0 |
14.0 ± 6.4 |
|||
| T98G |
5.5 ± 1.9 |
50.2 ± 7.7 |
22.4 ± 3.0 |
14.3 ± 1.4 |
27.7 ± 9.5 |
30 ± 8** |
|
| T98G + IFNA2c |
5.7 ± 5.3 |
61.9 ± 14.8 |
14.3 ± 6.0 |
6.9 ± 1.6 |
|||
| MDA-MB-231 |
1.3 ± 0.3 |
46.0 ± 5.5 |
19.8 ± 2.3 |
22.9 ± 2.6 |
9.5 ± 2.1 |
23 ± 5*** |
|
| MDA-MB-231 + IFNA2c |
1.1 ± 0.6 |
62.8 ± 12.7 |
17.5 ± 3.0 |
18.2 ± 1.8 |
|||
| Group 2 |
HeLa S3 |
1.6 ± 1.4 |
53.3 ± 6.2 |
20.2 ± 1.7 |
16.9 ± 1.6 |
27.6 ± 3.6 |
54 ± 11*** |
| HeLa S3 + IFNA2c |
0.9 ± 1.1 |
37.7 ± 7.6 |
33.7 ± 6.6 |
20.4 ± 4.9 |
|||
| BJAB |
0.3 ± 0.4 |
51.2 ± 10.8 |
24.6 ± 6.8 |
17.8 ± 10.3 |
5.3 ± 1.4 |
42 ± 5** |
|
| BJAB + IFNA2c |
0. Nine ± 1.4 |
30.8 ± 8.2 |
42.5 ± 12.6 |
13.8 ± 2.0 |
|||
| A549 |
0.9 ± 0.4 |
56.7 ± 3.8 |
20.4 ± 1.0 |
16.6 ± 9.4 |
10.5 ± 0.7 |
24 ± 7* |
|
| A549 + IFNA2c |
0.4 ± 0.2 |
38.3 ± 5.3 |
26.6 ± 6.5 |
17.8 ± 2.6 |
|||
| Group 3 | U937 |
2.2 ± 2.0 |
53.6 ± 3.5 |
23.9 ± 1.6 |
17.9 ± 2.1 |
8.7 ± 5.3 | 8 ± 6 ns |
| U937 + IFNA2c | 2.1 ± 1.4 | 49.1 ± 9.2 | 28.1 ± 1.6 | 17.7 ± 4.2 | |||
Different cell lines were incubated in the presence or absence of IFNA2c (3.6 ng/mL) for 48 h. To study changes in cell cycle, cells were stained with propidium iodide (PI), and signals were measured using a Cellometer image-based cytometer. To measure autophagic signals in living cells, Cellometer image-based cytometry in combination with Cyto-ID Green dye was used. To determine inhibition of proliferation, cell numbers were counted using Cellometer image-based cytometer in combination with Trypan Blue staining. Phase populations were reported as Apoptotic, G0/G1-, S- or G2/M-phase. Data shown are averages of three individual experiments, ± SD of experimental replicates. For inhibition of proliferation, we determined two-tailed p values by using a paired t-test that compared each treatment group relative to untreated control. Statistical significance was reported as follows: *p < 0.05 (significant); **p < 0.01 (very significant); ***p < 0.001 (extremely significant); ns: p > 0.05 (not significant).