Figure 1. Comparative effect of silibinin on viability of human CRC cells. (A) Effect of mitogens on silibinin-induced cell death in different human CRC cells under serum-starved (SS) conditions. (B) Effect of silibinin on viability, apoptotic death, cell cycle distribution and clonogenic potential of SW480 cells cultured under serum conditions. (C) Representative photomicrographs of SW480 cells cultured under different serum conditions, depicting intense cytoplasmic vacuolization due to silibinin treatment. All experimental procedures and statistical analysis were performed as detailed in Materials and Methods. ^#p < 0.01; *p < 0.001.