Figure 5. Visualization of autophagy induction and cellular morphology under transmission electron microscopy (EM) after treatment of SW480 cells with silibinin alone or cotreatment with antioxidant AA, or autophagy inhibitors 3MA, and BAFA1 under serum conditions. All experimental procedures and statistical analysis were performed as detailed in Materials and Methods. N, nucleus; C, cytoplasm; ER, endoplasmic reticulum with ribosomes as beads on membrane; M, mitochondria; AC, autophagic compartment; DAC, electron dense autophagic compartment; AP, autophagosomes.