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. 2013 May 31;8(5):e65040. doi: 10.1371/journal.pone.0065040

Figure 1. DDX5 is important for HIV-1 replication.

Figure 1

A. Fluorescence images show 293T cells co-transfected with pNL4-3ΔEnv-GFP and pcDNA3.1-DDX5-HA (or pcDNA3.1) at 48 h p.t. B. Western blotting analysis of DDX5 expression in 293T cells. C, D and E. HIV-1 p24 ELISA assay of 293T cells supernatants. 293T cells in 24-well plates were co-transfected with pNL4-3ΔEnv-GFP (or pCMVΔR8.2, pNL4-3) and differing amounts of pcDNA3.1-DDX5-HA (pcDNA3.1 as a control). The supernatants were collected at 48 h p.t. for assay of p24 ELISA. F. The effect of DDX5 knockdown on HIV-1 p24 production. Top, the supernatants from human primary CD4+T cells infected with pNL4-3 in the presence of DDX5-siRNA (GFP-siRNA as a control) were collected at 5 days post-infection and analyzed with p24 ELISA kit. Bottom, the effect of DDX5-siRNA in human primary CD4+T cells was detected by Western blotting. Data in C, D, E and F represent mean ±S.D. (error bars).