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. 2013 May 31;8(5):e65040. doi: 10.1371/journal.pone.0065040

Figure 3. DDX5 modulates Rev/RRE- but not CTE-dependent reporter gene expression.

Figure 3

A. Schematic maps of pMDLg/CTE and pMDLg/RRE. B. The 293T cells were co-transfected with pMDLg/RRE (or pMDLg/CTE), pcDNA3.1-Rev and pcDNA3.1-DDX5-HA (pcDNA3.1 as a control). C. The 293T cells were co-transfected with pMDLg/RRE (or pMDLg/CTE) and pcDNA3.1-Rev in the presence of DDX5-siRNA (GFP-siRNA as a control). B and C. The supernatants were collected for assay of HIV-1 p24 ELISA at 48 h p.t. Western blotting analysis was performed to confirm p55 Gag expression with anti-p24 antibody. The results are mean values with ±S.D. from triplicate samples.