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. 2013 May 31;8(5):e65747. doi: 10.1371/journal.pone.0065747

Figure 4. Premature and transient induction of genes regulated by σE.

Figure 4

Cells in which a ribosome maturation factor or a ribosomal protein was removed (A–C) and wild-type cells treated with 80 µg/ml kasugamycin, 1.2 µg/ml chloramphenicol or 2.5 µg/ml streptomycin (D) were used. Levels of rpoE mRNA (A and D), rpoH mRNA (B) and MicA (C) at the indicated time points after salt shock were analyzed by qRT-PCR. Total RNA fraction prepared from each of the cells was used as the template.