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. 2013 May 31;8(5):e65616. doi: 10.1371/journal.pone.0065616

Figure 4. Gal-9 treatment of resting PBMCs shifts T-cells towards central memory and T-helper 1 phenotype.

Figure 4

A−C. PBMCs were treated for 7 days with Gal-9 or Gal-9(S) after which the percentage of naïve (A), central memory (B), and effector memory (C) was evaluated by flow cytometry. D−G. Resting PBMCs were treated for 7 days with Gal-9 or Gal-9(S), after which T-cell cytokine production was analyzed by flow cytometry as described in M&M. The percentage of IL-2 (n = 11) (D), IFN-γ (E), IL-17 (F) and IL-4 (G) was determined. H. Supernatants of medium and Gal-9 (15 nM) treated PBMCs was harvested at day7 after which the amount of secreted IFN-γ and IL-17 was determined with ELISA. I. PBMCs (n = 11) were treated for 7 days with Gal-9 or Gal-9(S) after which the percentage of regulatory T-cells was determined. Unless indicated otherwise; (n = 12).