Figure 5. Absence of store-operated Ca²⁺ entry blocks Treg cell development.

(A) Staining of Foxp3 versus CD25 on CD4-positive thymocytes in neonates at day 2 after birth (left) and frequency (upper right) and absolute cell number (lower right) of each population. Error bars denote mean ± SEM. n=6. *, P<0.002, **, P<0.01; NS, not significant. (B) Foxp3 expression in Foxp3⁺CD25⁺ cells (population 3) from control (black lines) and DKO (grey lines) mice. (C) In vitro differentiation of Treg cell precursors into Foxp3⁺CD25^high Treg cells. (D) Proliferation of Foxp3⁺CD25⁺ cells from control (black lines) and DKO (grey lines) mice was assessed by Ki-67 staining. (E) Expression of IL-2 receptor components in Foxp3⁺CD25⁺ from control (black lines) and DKO (grey lines) mice. Data are representative of two (A, C and E) and more than three (B and D) independent experiments. See also Figure S3.