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. Author manuscript; available in PMC: 2014 May 23.
Published in final edited form as: Immunity. 2013 Mar 14;38(5):881–895. doi: 10.1016/j.immuni.2013.02.008

Figure 7. Efficient agonist-T cell development requires full activation of NFATs.

Figure 7

(A) Ca2+ influx in response to anti-CD3 crosslinking (n=3) in wild-type CD4+CD25hiFoxp3- (Treg precursors, solid line), CD4loCD8lo (Population including iNKT and pre-IELs) and DP thymocytes (conventional T cells). Error bars denote mean ± SEM. n=3. *, P<0.05; **, P=0.07. (B and C) Flow cytometric analysis of agonist-selected T-lineage cells (B) and conventional T cell development (C) in NFAT1 and NFAT2-doubly deficient mice. Frequencies represent Foxp3+CD25hi Treg cells in CD4SP thymocytes or in splenic CD4+TCRβ+ cells, iNKT cells in CD19- liver mononuclear cells, IEL precursors in total thymocytes or CD8αα+ IELs in intestinal TCRαβ+ T cells. Data are representative of two (B and C) and three (A) independent experiments. Error bars denote mean ± SEM. n=4. *, P<0.05; NS, not significant. See also Figure S5.