Table 1.
Primary antibodies and conditions for immunohistochemistry
| Marker | Source | Antibody Identifier | Dilution | Conditions |
|---|---|---|---|---|
| AE1/AE3 | Novus Biologicals | Cat#NB600-1322 | 1:40 | HIER, citrate buffer (pH 6.0) |
| p63 | Neomarkers Company | Cat#MS-1081-P | 1:200 | HIER, citrate buffer (pH 6.0) |
| CK5/6 | Dako | Cat#M7237 | 1:100 | HIER, citrate buffer (pH 6.0) |
| EGFR | Abcam | Cat#ab2430 | 1:100 | HIER, citrate buffer (pH 6.0) |
| pEGFR | Abcam | Cat#ab40815 | 1:500 | HIER, citrate buffer (pH 6.0) |
| GFAP | Abcam | Cat#ab16997 | 1:100 | HIER, citrate buffer (pH 6.0) |
| cKIT | Dako | Cat#A4502 | 1:100 | HIER, citrate buffer (pH 6.0) |
| S100 | Dako | Cat#Z0311 | 1:4000 | HIER, citrate buffer (pH 6.0) |
| alpha-SMA | Sigma | Cat#A2547 | 1:2000 | None |