Table 3.

Immunohistochemical markers and staining parameters

Marker	Tumor Staining	Notes
AE1/AE3	+++	Diffuse intense cytoplasmic staining of tumor cells; Adjacent normal gland had strong cytoplasmic staining of ducts and less intense staining of acini.
p63	+++	Diffuse nuclear staining in tumor cells; Adjacent normal gland had nuclear staining in periductular cells and in some cells between acini.
CK5/6	++	Most tumors (>50%) had cytoplasmic staining that was more readily detected in the spindled tumor population than in the polygonal tumor cells; normal salivary gland had multifocal staining in periductal and less commonly periacinar stromal cells.
EGFR	++	Tumor cells showing patchy to coalescing staining; Adjacent gland had moderate staining in the ducts.
pEGFR	+/++	Tumor cells showing patchy to coalescing staining; Adjacent gland had weak multifocal staining in ducts
GFAP	Rare solitary +	Mostly negative with very rare solitary tumor cell immunostaining; Adjacent tissue had staining in nerves
cKIT	Neg	Tumor cells were negative; Ducts in adjacent glands had weak staining
S100	Neg	Tumor was negative; Adjacent normal tissue had staining in peripheral nerves and moderate staining of salivary gland duct epithelium.
alpha-SMA	Neg	Negative in tumor cells.

Scoring of tumor immunostaining: “Neg” - none; “+” rare to 33% of tumor cells; “++” ~34% to 66% of tumor cells; “+++” ~67% to diffuse cellular immunostaining