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. 2013 May 31;8(5):e65224. doi: 10.1371/journal.pone.0065224

Figure 5. Biofilm semi-static model using confocal laser scanning microscopy (CLSM).

Figure 5

Biofilms were grown in TSBg on coverslips coated with poly-L-lysin for (A) 24h and (B) 72h, including E1162 wild-type strain (1), esp mutant E1162Δesp (2), the esp complemented strain E1162Δesp+esp (3), ebrB mutant E1162ΔebrB (4) and ebrB complemented strain E1162ΔebrB+ebrb (5). (C) Quantification of the average thickness and total biomass of biofilms using Comstat/Matlab analysis. Asterisks represent significant differences (*p<0.01) as determined by an unpaired two-tailed Student's t-test) between the indicated samples. Pictures were taken at 63×magnification with 2.5 optical zoom.