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. 2013 May 31;8(5):e65132. doi: 10.1371/journal.pone.0065132

Figure 4. AtMYB61-mediated activation of promoter activity in Saccharomyces cerevisiae in an AC dependent fashion.

Figure 4

(A) The sequence of the oligonucleotides cloned into the reporter vector using EcoRI and SalI sites. Each AC element or mutated ACI element is triplicated within the segment. (B) Schematic representations of the Effector (pYES2TRP::AtMYB61) and Reporter (pLacZi::AC) constructs used in this assay (CYC1: minimal yeast promoter). (C) Quantitative analysis of β-galactosidase activity in yeast after induction. The measurements in liquid assay were made from three biological independent replicates. Activation of artificial genes comprising a minimal CYC1 promoter fused to a tandem AC element or mutated ACI element upstream of the lacZ gene by AtMYB61 protein, upon growth of the yeast in galactose (light grey bars), gave rise to β-galactosidase activity that was significantly greater than the controls, as determined by analysis of variance (P<0.005); including each vector alone, or both together after growth on non-inducing glucose (dark grey bars). Error bars represent standard deviations. *indicates statistically significant, P<0.005, determined by t-test. Underlined bases corresponds to a substituted guanine.