Table 3. Kinetic properties of FoxLOX.

Substrate	KM [µM]	Kcat [min−1]	Kcat/KM [min−1M−1×106]
18∶2(n-6)	6.89±0.79	155.66±7.22	22.59
18∶3(n-3)	31.69±3.79	93.93±3.21	2.96
18∶3(n-6)	34.84±5.38	1114.64±102.76	31.99
20∶4(n-6)	3.37±0.55	102.57±3.79	30.44

Kinetic constants were spectrophotometrically analyzed by determining the initial rate of product formation at 234nm at different substrate concentrations. All experiments were performed in 50 mM Tris/HCl pH 8.0 at room temperature using a Varian Cary 300. The results are presented as mean values ± standard deviation derived from triplicate measurements.