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. 2013 May 31;8(5):e65163. doi: 10.1371/journal.pone.0065163

Figure 7. TGFβ1-dependent phosphorylation of 14-3-3σ regulates Smad3 dependent transcription through involvement of p53 in the transcriptional complexes.

Figure 7

(A) Results of the luciferase activity assay using CAGA (12)-luc reporter. Co-expression of p53 had a restrictive effect on Smad3-dependent transcriptional activity in cells not treated with TGFβ, * - p value<0.05. (B) The results of DNA immunoprecipitation analysis. p53 phosphorylated at Ser392 is recruited to the CAGA element in presence of 14-3-3σ and Smad3 in the manner dependent on 14-3-3σ phosphorylation. Densitometry analysis of the p53 precipitation is shown in accompanying graph. (C) Schematic presentation of the role of 14-3-3σ phosphorylation on a functional interaction of TGFβ1/Smad3 and p53/p21 signaling.