Table 1. Proteins identified as changing their phosphorylation upon TGFβ1 treatment.
| No a) | Protein Identity b) | Accesion No. b) | Probability b) | Est'd Z b) | Sequence coverage, % | Theoretical value | Experimental value | Matched peptides | ||
| pI | Mr(kDa) | pI | Mr(kDa) | |||||||
| 1 | Heat shock 70 kDa protein 8 isoform 2 | NP_69488.1 | 1.0e+000 | 2.16 | 36 | 5.6 | 53.61 | 4.8 | 60 | 15 |
| 2 | Heat shock 70 kDa protein 9B (mortalin-2) | AAH30634.1 | 1.0e+000 | 2.28 | 34 | 6.0 | 74.12 | 5.0 | 60 | 21 |
| 3 | Mitosis-specific chromosome segregation protein SMC1 homolog | AAB34405.1 | 1.0e+000 | 2.28 | 18 | 7.6 | 143.81 | 6.5 | 47.5 | 17 |
| 4 | Keratin 14 | AAP35850.1 | 1.0e+000 | 2.39 | 38 | 5.1 | 51.92 | 4.8 | 47 | 27 |
| 5 | ENO-1 | AAH50642.1 | 1.0e+000 | 1.98 | 35 | 7.0 | 47.49 | 7.5 | 45 | 13 |
| 6 | ENO-1 | AAH50642.1 | 1.0e+000 | 2.15 | 42 | 7.0 | 47.49 | 7.4 | 45 | 18 |
| 7 | Eukaryotic translation initiation factor 3, subunit 5 epsilon (EIF3S5) | AAP35540.1 | 1.0e+000 | 1.97 | 27 | 5.2 | 37.13 | 4.7 | 37 | 10 |
| 8 | ENO-1 | AAH50642.1 | 1.0e+000 | 2.08 | 49 | 7.0 | 47.49 | 7.3 | 45 | 19 |
| 9 | Keratin 5 | AAH71906.1 | 9.6e−001 | 1.45 | 24 | 7.7 | 62.59 | 7.8 | 40 | 15 |
| 10 | A+U-rich element RNA binding factor | BAA22860.1 | 1.0e+000 | 2.43 | 29 | 8.9 | 30.34 | 7.0 | 32 | 10 |
| 11 | Annexin A2, isoform 2 | AAH23990.1 | 1.0e+000 | 2.30 | 35 | 7.7 | 38.79 | 7.2 | 32 | 15 |
| 12 | CNN2 (calponin 2) | CAG46609.1 | 1.0e+000 | 1.04 | 24 | 7.0 | 34.09 | 7.0 | 32 | 10 |
| 13 | Keratin 10 | AAA59199.1 | 1.0e+000 | 1.70 | 18 | 4.7 | 39.84 | 8.0 | 26 | 9 |
| 14 | Peroxiredoxin 6 | NP_004896.1 | 1.0e+000 | 1.44 | 37 | 6.0 | 25.13 | 6.5 | 25 | 8 |
| 15 | Peroxiredoxin 1 | CAI13096.1 | 1.0e+000 | 2.26 | 44 | 6.4 | 19.13 | 9.0 | 19 | 10 |
| 16 | Keratin 14 | NP_000517.2 | 1.0e+000 | 2.35 | 40 | 5.1 | 51.89 | 4.6 | 45 | 26 |
| 17 | Keratin 17 | NP_000413.1 | 1.0e+000 | 2.32 | 39 | 5.0 | 49.37 | 4.3 | 40 | 23 |
| 18 | Laminin-binding protein | CAA43469.1 | 9.7e−001 | 0.69 | 20 | 4.8 | 31.89 | 4.0 | 33 | 6 |
| 19 | RAB6-interacting protein 2 (RAB6IP2) | AAH68006.1 | 9.4e−001 | 0.61 | 19 | 6.8 | 71.88 | 4.8 | 33 | 14 |
| 20 | Eukaryotic translation initiation factor 3, subunit 2 beta (EIF3S2) | NP_003748.1 | 1.0e+000 | 2.22 | 25 | 5.4 | 36.88 | 5.2 | 33 | 14 |
| 21 | Radical S-adenosyl methionine and flavodoxin domains 1 (RSAFD1) | AAH51888.1 | 9.9e−001 | 0.70 | 17 | 5.8 | 44.08 | 5.0 | 32 | 8 |
| 22 | Eukaryotic translation initiation factor 3, subunit 2 beta (EIF3S2) | NP_003748.1 | 1.0e+000 | 1.68 | 39 | 5.4 | 36.88 | 4.2 | 32 | 13 |
| 23 | Keratin 10, typeI, cytoskeletal | KRHU0 | 1.0e+000 | 1.87 | 26 | 5.2 | 59.74 | 5.2 | 26 | 17 |
| 24 | Zinc finger protein 62 homolog | NP_689496.1 | 1.0e+000 | 0.86 | 16 | 9.9 | 58.78 | 8.3 | 20 | 9 |
| 25 | RAB6-interacting protein 2 isoform alpha | NP_055879.1 | 6.3e−001 | 0.74 | 15 | 6.2 | 108.89 | 8.3 | 20 | 14 |
| 26 | Antigen MLAA-34 | AAQ93064.1 | 9.0e−001 | 0.93 | 26 | 8.7 | 39.24 | 8.0 | 18 | 8 |
| 27 | Eukaryotic translation initiation factor 4H, isoform 2 | AAH10021.1 | 1.0e+000 | 2.19 | 44 | 7.8 | 25.24 | 8.0 | 25 | 9 |
| 28 | Keratin 10, type I, cytoskeletal | KRHU0 | 1.0e+000 | 1.78 | 22 | 5.2 | 59.74 | 9.5 | 20 | 12 |
| 29 | Heterogeneous nuclear ribonucleoprotein A2/B1 isoform A2 | NP_002128.1 | 1.0e+000 | 1.84 | 20 | 8.7 | 36.05 | 9.2 | 30 | 8 |
| 30 | Heterogeneous nuclear ribonucleoprotein A2/B1 isoform A2 | NP_002128.1 | 1.0e+000 | 2.36 | 48 | 8.7 | 36.05 | 9.3 | 30 | 13 |
| 31 | Heterogeneous nuclear ribonucleoprotein A2/B1 isoform A2 | NP_002128.1 | 1.0e+000 | 2.18 | 40 | 8.7 | 36.05 | 9.0 | 32 | 15 |
| 32 | Heterogeneous nuclear ribonucleoprotein A2/B1 isoform A2 | NP_002128.1 | 1.0e+000 | 2.31 | 29 | 8.7 | 36.05 | 9.2 | 29 | 11 |
| 33 | Heterogeneous nuclear ribonucleoprotein A2/B1 isoform A2 | NP_002128.1 | 1.0e+000 | 2.38 | 42 | 8.7 | 36.05 | 8.7 | 28 | 12 |
| 34 | Heterogeneous nuclear ribonucleoprotein A2/B1 isoform A2 | NP_002128.1 | 1.0e+000 | 1.09 | 15 | 8.7 | 36.05 | 8.7 | 25 | 7 |
| 35 | Human Muscle Fructose 1.6-Biphosphate Aldolase Complexed with Fructose 1,6-Biphosphate | 4ALD | 1.0e+000 | 2.09 | 28 | 8.8 | 39.73 | 8.8 | 35 | 10 |
| 36 | Human Muscle Fructose 1.6-Biphosphate Aldolase Complexed with Fructose 1,6-Biphosphate | 4ALD | 1.0e+000 | 2.25 | 24 | 8.8 | 39.73 | 9.1 | 35 | 9 |
| 37 | Human Muscle Fructose 1.6-Biphosphate Aldolase Complexed with Fructose 1,6-Biphosphate | 4ALD | 1.0e+000 | 1.28 | 22 | 8.8 | 39.73 | 9.0 | 35 | 8 |
| 38 | Vasodilator-stimulated phosphoprotein | CCA67147.2 | 1.0e+000 | 2.15 | 26 | 9.2 | 39.76 | 9.5 | 45 | 6 |
| 39 | Vasodilator-stimulated phosphoprotein | CCA67147.2 | 1.0e+000 | 1.77 | 21 | 9.2 | 39.76 | 9.3 | 45 | 11 |
| 40 | Keratin 10, type I, cytoskeletal | KRHU0 | 8.6e−001 | 0.60 | 13 | 5.2 | 59.74 | 7.2 | 46 | 8 |
| 41 | ENO 1 | AAH50642.1 | 1.0e+000 | 2.35 | 43 | 7.0 | 47.49 | 6.8 | 38 | 19 |
| 42 | ENO 1 | AAH50642.1 | 1.0e+000 | 2.40 | 35 | 7.0 | 47.49 | 7.0 | 38 | 14 |
| 43 | Inosine monophosphate (IMP) dehydrogenase 2 | AAH155567.1 | 9.7e−001 | 0.69 | 12 | 6.4 | 56.24 | 6.5 | 47 | 8 |
| 44 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 | NP_055555.1 | 9.9e−001 | 0.79 | 20 | 6.3 | 47.25 | 6.3 | 47 | 7 |
| 45 | Stress-induced phosphoprotein 1 (Hsp70/Hsp90-organining protein) | AAV38814.1 | 1.0e+000 | 1.98 | 31 | 6.4 | 63.25 | 6.5 | 50 | 20 |
| 46 | Stress-induced phosphoprotein 1 (Hsp70/Hsp90-organining protein) | AAV38814.1 | 1.0e+000 | 1.98 | 33 | 6.4 | 63.25 | 6.7 | 50 | 20 |
| 47 | Fumarate hydratase | AAP88841.1 | 1.0e+000 | 1.32 | 17 | 9.0 | 54.79 | 7.5 | 40 | 9 |
| 48 | Kerain 10, type I, cytoskeletal 10 | K1CJ | 1.0e+000 | 1.14 | 13 | 5.1 | 59.73 | 7.2 | 40 | 9 |
| 49 | Zinc finger protein 62 homolog | NP_689496.1 | 9.7e−001 | 0.63 | 19 | 9.9 | 58.78 | 7.8 | 35 | 9 |
| 50 | Antigen MLAA-34 | AAQ93064.1 | 9.7e−001 | 0.61 | 18 | 8.7 | 39.24 | 6.8 | 30 | 6 |
| 51 | Cytokeratin 9 | AAC60619.1 | 1.0e+000 | 1.83 | 23 | 5.1 | 62.20 | 6.5 | 33 | 8 |
| 52 | RNA binding protein 4 | AAH32735.1 | 1.0e+000 | 1.94 | 23 | 6.6 | 40.70 | 6.6 | 33 | 9 |
| 53 | Keratin 9 | NP_000217.2 | 1.0e+000 | 2.18 | 26 | 5.1 | 62.27 | 6.9 | 33 | 14 |
| 54 | Qntigen MLAA-34 | AAQ93064.1 | 9.8e−001 | 0.68 | 23 | 8.7 | 39.24 | 7.3 | 27 | 6 |
| 55 | Zinc finger protein 62 homolog | NP_689496.1 | 1.0e+000 | 0.99 | 18 | 9.9 | 58.78 | 7.2 | 26 | 8 |
| 56 | Guanyl nucleotide releasing protein 4 | AAL87858.1 | 9.4e−001 | 0.51 | 9 | 9.1 | 76.22 | 7.5 | 27 | 9 |
| 57 | Transcription factor NRF | AAH47878.1 | 9.6e−001 | 0.57 | 11 | 9.2 | 78.34 | 7.9 | 28 | 7 |
| 58 | Heterogeneous nuclear ribonucleoprotein A2/B1 isoform A2 | NP_002128.1 | 1.0e+000 | 2.31 | 34 | 8.7 | 36.05 | 7.9 | 30 | 11 |
| 59 | Heat shock 70 kDa protein 9B (mortalin-2) | AAH30634.1 | 1.0e+000 | 2.26 | 41 | 6.0 | 74.12 | 5.0 | 48 | 25 |
| 60 | Heat shock 70 kDa protein 9B (mortalin-2) | AAH30634.1 | 1.0e+000 | 2.26 | 33 | 6.0 | 74.12 | 4.5 | 60 | 19 |
| 61 | Heat shock 70 kDa protein 9B precursor (MTHSP75) | AAA67526.1 | 1.0e+000 | 2.37 | 18 | 6.0 | 74.05 | 5.0 | 50 | 14 |
| 62 | Heat shock 70 kDa protein 9B (mortalin-2) | AAH30634.1 | 1.0e+000 | 1.71 | 17 | 6.0 | 74.12 | 5.3 | 50 | 11 |
| 63 | Unknown (chaperonin containing TCP1) | AAH02971.1 | 1.0e+000 | 1.99 | 23 | 5.4 | 59.91 | 5.2 | 47 | 12 |
| 64 | Prolyl 4-hydroxylase, beta subunit | NP_000909.2 | 1.0e+000 | 2.28 | 32 | 4.8 | 57.50 | 4.0 | 47 | 17 |
| 65 | Chaperonin containing TCP1, subunit 2 (beta) | AAI13517.1 | 1.0e+000 | 0.86 | 16 | 9.9 | 58.78 | 8.3 | 20 | 10 |
| 66 | Tubulin, beta polypeptide | NP_821133.1 | 1.0e+000 | 2.39 | 38 | 4.8 | 50.11 | 4.3 | 46 | 34 |
| 67 | Keratin 7 | NP_005547.2 | 1.0e+000 | 2.39 | 35 | 5.4 | 51.46 | 5.0 | 46 | 17 |
| 68 | Heterogeneous nuclear ribonucleoprotein K isoform a | NP_112553.1 | 1.0e+000 | 2.32 | 28 | 5.2 | 51.30 | 7.5 | 46 | 13 |
| 69 | Gamma-actin | JC5818 | 1.0e+000 | 1.80 | 35 | 5.3 | 41.99 | 5.0 | 35 | 10 |
| 70 | Ribosomal protein P0 | AAH01127.1 | 1.0e+000 | 1.98 | 34 | 5.4 | 34.43 | 5.3 | 31 | 10 |
| 71 | Ribosomal protein P0 | AAH01127.1 | 1.0e+000 | 1.98 | 34 | 5.4 | 34.43 | 5.5 | 31 | 9 |
| 72 | SFN (14-3-3 sigma, stratifin) | AAH01550.1 | 1.0e+000 | 2.18 | 37 | 4.8 | 24.38 | 6.5 | 32 | 10 |
| 73 | Eukaryotic translation elongation factor 1 beta 2 | AAP35742.1 | 1.0e+000 | 1.54 | 29 | 4.5 | 24.92 | 3.7 | 25 | 8 |
| 74 | SFN (14-3-3 sigma, stratifin) | AAH01550.1 | 1.0e+000 | 2.18 | 45 | 4.8 | 24.38 | 4.2 | 24 | 21 |
| 75 | Mitochondrial short-chain enoyl-coenzyme A hydratase 1 precursor | NP_004083.2 | 1.0e+000 | 2.29 | 50 | 8.9 | 31.81 | 6.0 | 24 | 16 |
| 76 | Proteasome alpha 5 subunit | NP_002781.2 | 1.0e+000 | 2.29 | 50 | 8.9 | 31.81 | 6.0 | 24 | 16 |
| 77 | Ribosomal protein S6 kinase, 90 kda, polypeptide 3 | BAC81131.1 | 1.0e+000 | 1.05 | 16 | 6.4 | 82.48 | 6.3 | 10 | 10 |
| 78 | Keratin9 | NP_00217.2 | 1.0e+000 | 1.14 | 18 | 5.1 | 62.27 | 9.0 | 10 | 14 |
| 79 | Chain B Human Mitochondrial Single Strand DNA Binding Protein (HMSSB) | 1S3O_B | 1.0e+000 | 1.98 | 49 | 8.2 | 15.18 | 9.2 | 8 | 7 |
| 80 | RAS guanyl releasing protein 4 | AAK85701.1 | 9.7e−001 | 0.63 | 11 | 9.0 | 11.79 | 9.0 | 7 | 10 |
| 81 | Fk506 Binding Protein Fkbp Mutant R42kH87V Complex With Immunosuppressant Fk506 | 1BKF_A | 1.0e+000 | 2.24 | 46 | 8.1 | 11.79 | 9.0 | 7 | 10 |
| 82 | Heat shock 70 kDa protein 8 isoform 1 | NP_006588.1 | 1.0e+000 | 2.37 | 32 | 5.4 | 71.11 | 4.1 | 63 | 19 |
| 83 | Eukaryotic translation elongation factor 2 | NP_001952.1 | 8.2e−001 | 0.64 | 13 | 6.4 | 96.29 | 3.9 | 25 | 11 |
| 84 | Endoplasmic reticulum protein 29 precursor | NP_006808.1 | 1.0e+000 | 2.26 | 34 | 6.8 | 29.03 | 6.2 | 25 | 10 |
| 85 | Lamin A/C isoform 2 | NP_005563.1 | 1.0e+000 | 2.31 | 35 | 6.4 | 65.17 | 6.6 | 49 | 18 |
a
)- selected phosphoprotein spots from 2-D gels;
b
)-NCBI sequence identification numbers.
Probability, Z-value, coverage and theoretical pI and Mr were obtained from ProFound search. The calculation of experimental pI and Mr was based on migration of proteins on 2-D gels.