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. 2013 May 31;8(5):e64374. doi: 10.1371/journal.pone.0064374

Figure 4. Obestatin up-regulates nestin expression at stage 4 and binds to enriched islets and MLCs.

Figure 4

(A) Nestin mRNA assessed by RT-PCR in control (c) and obestatin-treated cells (Obe). Buffer alone was used as negative control (–). Shown is a representative image of three independent experiments. (B) Nestin protein expression determined by Western blot in lysates cultures that were either untreated or treated with obestatin (n = 3). (C) TAMRA-Obestatin (T-Obe) (1 µM) binding to adherent islets and emerging MLCs. The image is representative of both untreated and obestatin-treated cultures, that were tested in at least three independent experiments (magnification, X20). (D-E) T-Obe (1 µM) binding to nestin+ neuronal-like cells (D) and to Nestin+ MLCs (E) in both control and obestatin-treated cultures (n = 3; magnification, X40). T-Obe is shown in red, nestin in green and nuclei in blue (DAPI).