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. 2013 May 31;8(5):e64404. doi: 10.1371/journal.pone.0064404

Figure 4. Loss of K7 is associated with hyperproliferation but not hyperplasia of the bladder urothelium.

Figure 4

Immunohistochemistry of bladder sections from wildtype (A, D), heterozygous (B, E) and homozygous K7 knockout mice (C, F) stained with a rabbit polyclonal antibody to K7 (A, B, C) and mouse monoclonal antibody MM1 to the cell proliferation marker Ki-67 (D, E, F). Arrowheads and insets in panels D and E indicate Ki-67 positive nuclei in wildtype (D) and heterozygous K7 knockout (E) bladder. More Ki-67 positive cell nuclei can be seen in the bladder of homozygous K7 knockout mice (arrowheads in F). Scale bars = 50 µm. G. Graph showing the percentage of Ki-67 positive urothelial cells in wildtype, heterozygous and homozygous K7 knockout mice (5 bladders per genotype). For each bladder, 10 random images were collected and an average of 1480 (SD +/−300) urothelial cell nuclei were counted. Standard errors (SE) are indicated by the capped lines. * indicates a p value of less than 0.05 (WT p = 0.01; HET p = 0.007). H. H&E stained sections of the bladder urothelium of wildtype, heterozygous and homozygous K7 knockout mice. Scale bar = 25 µm.