Figure 1. Relationships between uptake of radiolabeled acetate, FASN expression, and sensitivity to orlistat treatment in vitro.

(A) Uptake of [1-¹⁴C]acetate (upper) and levels of FASN expression (lower) in LNCaP, PC3, 22Rv1, and DU145 cells. The groups with different alphabets are significantly different (P<0.05). (B) Percent cell viability after orlistat treatment in LNCaP, PC3, 22Rv1, and DU145 cells. Cell viability is indicated as a percentage of that with 0 µM orlistat treatment. Asterisks indicate statistical significance versus treatment with 0 µM for each cell line (*P<0.05). (C) Relationship between uptake of [1-¹⁴C]acetate and FASN expression (upper), relationship between % cell viability after orlistat treatment at 12.5 µM and uptake of [1-¹⁴C]acetate (middle), and relationship between % cell viability after orlistat treatment at 12.5 µM and FASN expression (lower). Values from six independent experiments are shown. Data are expressed as means ± SD.