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. 2013 May 31;8(5):e64544. doi: 10.1371/journal.pone.0064544

Figure 4. Lentiviral transduction of BCL2A1-shRNA in SUDHL-1 cells and its influence on cell proliferation and apoptosis.

Figure 4

(A) Flow cytometric analysis of transduced SUDHL-1 cells and untreated controls three days after infection. The percentage of GFP-positive cells represents the percentage of infected cells. (B) RT-qPCR analysis of BCL2A1 mRNA in the transduced SUDHL-1 cells three days after infection. Values were normalized to TBP and data were analyzed according to the 2−ΔΔCp method. Results are depicted as mRNA amount relative to untreated SUDHL-1 cells. Error bars indicate SD (n = 3). (C) Proliferation curves of the controls and BCL2A1-shRNA infected SUDHL-1 cells up to 5 days after infection. Error bars indicate SD (n = 3). (D) Annexin V/propidium iodide staining of the controls and the BCL2A1-shRNA transduced SUDHL-1 cells three days after infection. SUDHL-1 = uninfected cells, pG-NS = pGIPZ vector with non-silencing shRNA, pG-BCL2A1 = virus containing the pGIPZ vector and the BCL2A1-shRNA sequence, PI = propidium iodide. (E) BCL2A1 immunostaining in ALCL. Representative ALK+ (left) and ALK- (middle) ALCL cases showing both a similar cytoplasmic BCL2A1 positivity in the tumor cells comparable to the expression in SUDHL-1 cells. (Immunoperoxidase, 400x).