Figure 3. Irs2-deficient neonatal mice have reduced testis size.

(A) Testes were fixed in Bouin's solution and embedded in paraffin, and sections were cut to a thickness of 5 µm and stained with hematoxylin and eosin. Images were captured at 100× magnification under oil immersion. The scale bar represents 20 µm. Representative images of testicular cross sections of WT and Irs2 ^−/− at P0 (day of birth) and P4. (B) Testes weights and (C) the testes weights to body weight ratio were reduced in Irs2-deficient mice at P0 and P4. (D) Diameter and (E) circumference of seminiferous cords were measured in WT and Irs2 ^−/− at P0 or P4. Asterisks denote a significant difference compared to WT; * P<0.05, ** P<0.01, *** P<0.001. (n = 4 male pups of each genotype).