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. 2013 May 31;8(5):e65309. doi: 10.1371/journal.pone.0065309

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© 2013 Yang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Silencing of AK126698 by siRNA in A549 cells and altered NKD2 mRNA expression in AK126698 knockdown A549 cells (A). Western blot analysis of β-catenin and phospho-β-catenin levels in AK126698 knockdown A549 cells (B). Apoptosis evaluated by flow cytometry in AK126698 knockdown A549 cells (C). Values are means±SD and obtained from three independent experiments. *P<0.05, ***P<0.001 compared with A549 and negative control. NC: A549 treated with siRNA negative control; AK126698 siRNA-424, A549 treated with siRNA AK126698-424; AK126698 siRNA-291, A549 treated with siRNA AK126698-291; P-β-catenin, phospho-β-catenin.