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. 2013 May 31;8(5):e64711. doi: 10.1371/journal.pone.0064711

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© 2013 Merlet et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Representative β₃-AR immunoblotting at days 35 (d35) and 70 (d70). B. β₃-AR protein quantification at day 35. C. β₃-AR protein quantification at day 70. Protein levels were quantified using Amersham ImageQuant RT-ECL camera (GE Healthcare). The band signals were assessed by densitometry with ImageQuant TL software (GE Healthcare) and a ratio to the corresponding GAPDH band intensity was calculated. D. Cardiac inotropic (dP/dt_max), E. lusitropic (dP/dt_min) and F. chronotropic (HR) effects of increasing concentrations of SR 58611A (10⁻⁹ to 10⁻⁵ M) were evaluated on isolated perfused hearts. **: P<0.001 vs Ctrl. Ctrl: control, Dox-CM: Doxorubicin-induced cardiomyopathy.