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. 2013 May 31;8(5):e64711. doi: 10.1371/journal.pone.0064711

Figure 5. Impaired β1-AR expression in Dox-CM hearts was not associated to an impaired cardiac β1-AR function.

Figure 5

A. Representative β1-AR immunoblotting at days 35 (d35) and 70 (d70). B. β1-AR protein quantification at day 35. C. β1-AR protein quantification at day 70. Protein levels were quantified using Amersham ImageQuant RT-ECL camera (GE Healthcare). The band signals were assessed by densitometry with ImageQuant TL software (GE Healthcare) and a ratio to the corresponding GAPDH band intensity was calculated. D. Cardiac inotropic (dP/dtmax), E. lusitropic (dP/dtmin) and F. chronotropic (HR) effects of increasing concentrations of isoproterenol (10−9 to 10−5 M) in the presence of 10−6 M ICI-118,551 a β2-AR antagonist and 10−6 M of L-748,337 a β3-AR antagonist were evaluated on isolated perfused hearts. B: Baseline; B+A; Baseline in presence of antagonists. **: P<0.001 vs Ctrl. Ctrl: control, Dox-CM: Doxorubicin-induced cardiomyopathy.