Table 1. Antibodies used for western-blot.
Primary antibody | Secondary antibody | |||
Protein | Description | Dilution | Description | Dilution |
β1-AR | Rabbit polyclonal antibody, Sigma-Aldrich(A-272) | 1/2,000 | Goat anti-rabbit immunoglobulin G, Santa-Cruz Biotechnology (sc-2054) | 1/10,000 |
β2-AR | Rabbit polyclonal antibody, AbCam Ltd(ab36956) | 1/2,000 | Goat anti-rabbit immunoglobulin G, Santa-Cruz Biotechnology (sc-2054) | 1/15,000 |
β3-AR | Rabbit polyclonal antibody, Santa CruzBiotechnology (sc-50436) | 1/5,000 | Goat anti-rabbit immunoglobulin G, Santa-Cruz Biotechnology (sc-2054) | 1/15,000 |
Gsα | Rabbit polyclonal antibody, Merk Chemicals Ltd(371732) | 1/1,000 | Goat anti-rabbit immunoglobulin G, Santa-Cruz Biotechnology (sc-2054) | 1/10,000 |
Giα2 | Rabbit polyclonal antibody, Merk Chemicals Ltd(371727) | 1/5,000 | Goat anti-rabbit immunoglobulin G, Santa-Cruz Biotechnology (sc-2054) | 1/10,000 |
GAPDH | Mouse monoclonal antibody, Santa-CruzBiotechnology (sc-32233) | 1/10,000 | Goat anti-mouse immunoglobulin G, Santa-Cruz Biotechnology (sc-2055) | 1/50,000 |
Primary antibodies were diluted in TBS-T, excepted for Giα2 protein detection which was diluted in 5% non-fat dry milk in TBS-T. All secondary antibodies were diluted in 1% non-fat dry milk in TBS-T.