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. 2013 May 31;8(5):e64711. doi: 10.1371/journal.pone.0064711

Table 1. Antibodies used for western-blot.

Primary antibody Secondary antibody
Protein Description Dilution Description Dilution
β1-AR Rabbit polyclonal antibody, Sigma-Aldrich(A-272) 1/2,000 Goat anti-rabbit immunoglobulin G, Santa-Cruz Biotechnology (sc-2054) 1/10,000
β2-AR Rabbit polyclonal antibody, AbCam Ltd(ab36956) 1/2,000 Goat anti-rabbit immunoglobulin G, Santa-Cruz Biotechnology (sc-2054) 1/15,000
β3-AR Rabbit polyclonal antibody, Santa CruzBiotechnology (sc-50436) 1/5,000 Goat anti-rabbit immunoglobulin G, Santa-Cruz Biotechnology (sc-2054) 1/15,000
Gsα Rabbit polyclonal antibody, Merk Chemicals Ltd(371732) 1/1,000 Goat anti-rabbit immunoglobulin G, Santa-Cruz Biotechnology (sc-2054) 1/10,000
Giα2 Rabbit polyclonal antibody, Merk Chemicals Ltd(371727) 1/5,000 Goat anti-rabbit immunoglobulin G, Santa-Cruz Biotechnology (sc-2054) 1/10,000
GAPDH Mouse monoclonal antibody, Santa-CruzBiotechnology (sc-32233) 1/10,000 Goat anti-mouse immunoglobulin G, Santa-Cruz Biotechnology (sc-2055) 1/50,000

Primary antibodies were diluted in TBS-T, excepted for Giα2 protein detection which was diluted in 5% non-fat dry milk in TBS-T. All secondary antibodies were diluted in 1% non-fat dry milk in TBS-T.