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. 2013 May 31;8(5):e64693. doi: 10.1371/journal.pone.0064693

Figure 3. Results of primary screen of an siRNA library targeting human druggable proteins to identify host factors involved in Toxoplasma invasion.

Figure 3

A. 2,742 genes were screened in triplicate replica plates using U-Luc parasites. The values obtained with NT siRNA controls were set at 100% invasion and the values for target genes were calculated relative to the NT siRNA. The black dotted line represents the mean of the non-targeting siRNA control and the red-dotted lines indicate the 3 standard deviations (SD) boundaries of the mean. Each green dot represents the mean percentage invasion from triplicate samples for one target gene. Only those that showed >90% host cell viability after silencing are shown. B. Table summarizing overall screen. A total of 2,742 genes were subjected to primary screen. Of these 300 genes showed more than >3SD inhibition or enhancement of invasion and >90% host cell viability. The top 100 genes showing inhibition were subjected to secondary screen by luciferase assay and 81 of these were thereby validated. When these genes were subjected to high-content red-green assay, 24 of these showed significant inhibition of parasite invasion.