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. 2013 May 31;8(5):e64723. doi: 10.1371/journal.pone.0064723

Figure 1. In vitro characterization of NCSCmix and MSCmix, isolated from adult Wnt1-Cre/R26R-LacZ mouse bone marrow.

Figure 1

BMSC were harvested from double-transgenic Wnt1-CRE/R26R-LacZ mouse bone marrow (A). Amplification of the PGK-Neo cassette confirmed that NCSCmix underwent recombination, conversely to MSCmix (B). NCSCmix were β-galactosidase positive (blue, C), whereas MSCmix were not (blue, K) (Hematoxylin–stained nuclei). NCSCmix expressed neural crest-associated proteins Sox10 (green, D), Nestin (green, E) and p75NTR (green, F). MSCmix were Sox10-negative (green, L), weakly p75NTR-positive (green, N), and only a small proportion (<15%) of cells expressed nestin (green, M). MSCmix also expressed Sca-1 (green, O) and CD24 (green, P) while NCSCmix did not (green, G-H), and both types of cells were positive for Fzd-4 (green, I-Q). In NCSCmix, some β-tubulin-expressing cells were detected (in MesenCult medium) (green, J), but no cell in the MSCmix was β-tubulin-positive in those conditions (green, R) (DAPI-stained nuclei). (Scale bars = 30 µm).