Figure 1. The total RNA, isolated from Human laryngeal squamous cell carcinoma radioresistant cell Hep2R, was used to synthesized the first-strand cDNA and double-strand cDNA by SMART method (Clontech).

The cDNA fragments were inserted into the pGADT7 vector, and the recombinant phage were packaged in vitro. A small portion packaged phage was used to infected DH10B Competent Cells. Titration and the positive clones were assayed by PCR. Fig. 1 shows the inserted fragment of Hep2R cell full length cDNA library detected through construction electrophoresis. Table 1 shows the proteins found through Y2H from Hep2R cell cDNA library.