Figure 6. The MCF-7 cells radiosensitivity detection were illustrated when exposed to irradiation, depending on doses in GY, MCF-7 cells transfected with pshRNA-UBE2D3 showed reductions of clonogenic survival compared to negative control.

Each group of cells were irradiated at the dose point of 0, 1, 2, 4, 6, 8, 10 GY respectively. After 14 days of incubation, the colonies were fixed and stained. Those colonies containing >50 cells were scored as viable colonies. The data were fit into the linear-quadratic model, and survival curve of each group were demonstrated by Graphpad prism 5.0 software. Each experiment was done at least three times in triplicate wells.