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. 2013 May 31;8(5):e64616. doi: 10.1371/journal.pone.0064616

Figure 5. Analysis of the histological structure of secondary immune structures in IUGRSal and IUGRLep piglets at d21.

Figure 5

(A) Histological structure of spleen and immunodetection of CD79+ cells in IUGRSal and IUGRLep piglets at d21. Left panels: Microscopic observation of white pulpe (W) in spleen samples processed with a hemalun-Eosin-Safran staining. Bars = 50 µm. Central arteriolae are indicated by arrows. Primary follicles were easily identified in IUGRLep (b) and delineated with the small circle but were not visible in IUGRSal (a). The inset in (b) illustrates cells undergoing intense mitotic activity (arrowhead) found in this area. Right panels: Immunolabelling for CD79 in the white pulp of IUGR piglets treated either with saline (c) or leptin (d) postnatally. (B) Histomorphometrical analysis of Peyer’s patches in IUGRSal and IUGRLep piglets at d21. The areas of the whole Peyer’s Patches (PP) and the B follicle were measured and the ratio corresponding to the B follicle area reported to the whole Peyer’s patche surface was determined. Values represent the mean ± SEM (n = 6 per group), *: p<0.05; **: p<0.01, for leptin effect in IUGR piglets.