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. 2013 May 31;8(5):e64689. doi: 10.1371/journal.pone.0064689

Figure 1. Degradation of SPLUNC1 protein by human neutrophil elastase (HNE).

Figure 1

(A) Western blot of recombinant human SPLUNC1 protein (10 µg/ml) that was incubated with HNE for 30 minutes. HNE treatment dose-dependently reduces the native SPLUNC1 protein (25 kD). A fragment of 22-kD SPLUNC1 was seen in HNE-treated samples. (B) Mass spectrometry analysis of NHE-treated recombinant human SPLUNC1 protein revealed a 25 amino acid peptide F21-T45 (FGGLPVPLDQTLPLNVNPALPLSPT).